Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

What is the life cycle type of bryophytes, What is the life cycle type of b...

What is the life cycle type of bryophytes? As in all plants the life cycle of bryophytes is diplobiontic (alternation of generations). In bryophytes the lasting form is the hap

Computed radiography, C omp ut e d radiography: The d...

C omp ut e d radiography: The development of intensifying screen has greatly improved the efficiency of exposure  and has reduced the exposure time.   The fir

Forms of ribosomes, FORM S OF RIBOSOMES (1 )      Bound form - Atta...

FORM S OF RIBOSOMES (1 )      Bound form - Attached to ER. Protein synthesis for secretion purpose occurs on bound ribosomes. (2 )      Free Ribosomes - Occur freely

Define limitations for standard or total plate counts method, Define Limita...

Define Limitations for Standard or Total Plate Counts Method? This method has certain limitations - 1. Microbial count depends on the type of media, incubation temperature,

Explain microtube and hedstorm file removal technique, Explain Microtube an...

Explain Microtube and Hedstorm file removal technique A Hedstorm file was then passed down the length of the tube until it engaged itself tightly between the obstruction an

Classification of multicellular animals - coelom, Classification of Multice...

Classification of Multicellular Animals - Coelom We know earlier that the pseudocoel gave animals specific selective benefits. Among other things, this fluid-tilled space wor

How to load glycogen, How to load glycogen? Over the past fifty years, ...

How to load glycogen? Over the past fifty years, the biggest breakthrough was the discovery of how to load glycogen and sophistication in the methods of glycogen loading. Nitro

Explain the heart rate monitoring (hrm) method, Explain the Heart Rate Moni...

Explain the Heart Rate Monitoring (HRM) Method? HRM is a method to measure the daily energy expenditure of free-living individuals, based on the relationship of heart rate and

Phase (different interference) contrast microscopy, Phase (different interf...

Phase (different interference) Contrast microscopy: Living cells are mostly   transparent. For viewing under ordinary light microscope, therefore, live cells must be stained wi

Explain functional properties of food lipids, Functional properties of food...

Functional properties of food lipids Chemically, fats and oils, as you may already know by now, are a combination of glycerol and fatty acids. The glycerol molecule has three s

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd