Define about the paper chromatography, Biology

Assignment Help:

Define about the Paper Chromatography?

Paper chromatography is a very useful technique for separating mixtures of metal ions, anions, amino acids, sugars, dyes, drugs, etc. The chief factor responsible for separation of ingredients by paper chromatography is partition. Any paper used should have space in the interstices. It is in the interstices, that molecules of one phase is embedded. Hence paper like Whatman No. l, 3 are used.

The paper functions as an inert support when the mobile phase moves along the paper, the substances applied as a spot on the paper are partitioned between the mobile solvent and molecules of the static solvent embedded in the interstices of the paper. As the partition coefficient may be different for different substances, the rates of migration of substances along the paper are also different and thus separation takes place.


Related Discussions:- Define about the paper chromatography

Seed formation, What are the 3 phases of seed formation

What are the 3 phases of seed formation

When does the greatest buffering capacity of weak acids, The greatest buffe...

The greatest buffering capacity of weak acids is when- Select one: a. They are almost completely dissociated b. They are almost completely undissociated c. They are nea

What is the conjugate acid, Calculate the pH: 300mL of 0.25M sodium ascorba...

Calculate the pH: 300mL of 0.25M sodium ascorbate plus 150mL of 0.2M HCl (the pKa of ascorbic acid is 4.04) okay, what's the conjugate acid and base of this problem? Can someone wa

Mixed mitral stenosis and regurgitation, Mixed Mitral Stenosis and Regurgit...

Mixed Mitral Stenosis and Regurgitation The most common cause for a combined lesion is rheumatic, Very rarely; it could be of congenital origin. Regurgitation in a stenotic val

Difference between artificial and natural selection, Difference between Art...

Difference between Artificial selection and natural selection - S.No Artificial selection Natural selection 1. It

Name the different systems of the body, The Different systems of the body a...

The Different systems of the body are: 1) Nervous System 2) Respiratory System 3) Cardiovascular System 4) Neurological and Sensory System 5) Gastrointestinal Syste

What is the advantage of the double and complete circulation, How many cham...

How many chambers do the bird heart and the mammalian heart have? Concerning temperature maintenance what is the advantage of the double and complete circulation of these animals?

5.4 assessment from the book, Briefly explain why cutting a flatworm into p...

Briefly explain why cutting a flatworm into pieces would not kill it

Almost bacteria are anaerobic, Most bacteria are anaerobic. If a bacterium ...

Most bacteria are anaerobic. If a bacterium were to produce the same amount of ATP within a minute as a cell extracted from your skeletal muscle, what would that bacterium have to

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd