cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Describe the process of rodent control, Q. Describe the process of Rodent c...

Q. Describe the process of Rodent control? Rats and mice are destructive and cause huge loss of stored food commodities. They transmit pathogenic bacteria. Rats and mice are ge

Vitamins, Which vitamin is useful for the clotting of blood?

Which vitamin is useful for the clotting of blood?

Abiotic factors of an ecosystem, Q. How does biological diversity relate to...

Q. How does biological diversity relate to the characteristics of the abiotic factors of an ecosystem? The ease of use of abiotic factors like light, mineral salts, moisture, h

Differences between genomes in prokaryotes and eukaryotes, What are the dif...

What are the differences between genomes in prokaryotes vs. eukaryotes?

Controlling expression of a number of genes in e, he Lac Repressor protein,...

he Lac Repressor protein, which we will talk about a bit later in the course, is important for controlling expression of a number of genes in E. coli. There are about 10 copies of

Bacteria, Bacteria The study of bacteria is called bacteriology. Bacte...

Bacteria The study of bacteria is called bacteriology. Bacteria are unicellular organisms and possess a distinct cell wall. They are included in kingdom Prokaryote. The bacter

Differences between ecosystems, Some ecosystems possess relatively large nu...

Some ecosystems possess relatively large number of species as compared to others. This is particularly true of tropical forests which exhibit a very rich-biodiversity. For example,

Explain boiling point - properties of solution, Boiling Point There are...

Boiling Point There are certain properties of solutions  which are directly connected with vapour pressure and one of it is boiling point.  You must have observed that water bo

Define specialized nutritional formulations for chd, Define Specialized Nut...

Define Specialized Nutritional Formulations for CHD? Achieving calorie and protein requirements with restricted fluid intake, you would realize, is a formidable challenge. Hig

Difference between cerebrum and brain, Q. What is the difference between ce...

Q. What is the difference between cerebrum and brain? What are the major parts of these structures? The concept of brain or encephalon comprehends the cerebrum mostly referred

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd