cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Determine about the trichromatic vision, Determine about the Trichromatic v...

Determine about the Trichromatic vision Trichromatic vision is the terminology used for normal vision, those who require aU' three primary colours to make a match with an unkno

Primary prevention - levels of prevention, Primary Prevention: Primary...

Primary Prevention: Primary prevention should have the following goals:  i)  Ascertaining at the risk population and  the high risk situations where stressful life events a

Applications in horticulture and agriculture, Applications in Horticulture ...

Applications in Horticulture and Agriculture While going round in public parks, you might have observed plants trimmed in such a way giving appearances of animals like camel,

Prokaryotic cell, whatis a prokaryotic cell? explain its structure and desc...

whatis a prokaryotic cell? explain its structure and description with images

The human impact on the environment, put the following events in the most p...

put the following events in the most probable order. A. Dead algae decomposed by bacteria B.excess nitrate and phosphate discharge into rivers C. Fish die of suffocation D.bacteria

Determine the food source for magnesium, Determine the food source for Magn...

Determine the food source for Magnesium? Magnesium is widely distributed in variety of foods and beverages. In plants it is associated with chlorophyll. Thus, green leafy veget

How you can repair ventricular aneurysm with a patch?, How you can Repair V...

How you can Repair Ventricular Aneurysm with a patch? Repair of Ventricular Aneurysm with a patch :  Originally described by Cooley, as endoaneurysmorra

What do you mean by platyhelminth phylum, Q What are the most tremendous kn...

Q What are the most tremendous known representatives of the platyhelminth phylum? The most popular representatives of the platyhelminthes are worms that cause human diseases, l

Define the criteria for an indicator organism, Define the Criteria for an I...

Define the Criteria for an Indicator Organism? Following criteria are suggested for an indicator organism: i. It should be suitable for the analysis of all types of water, i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd