cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What is the phenomenon of apical dominance in plants, What is the phenomeno...

What is the phenomenon of apical dominance in plants? How can it be artificially eliminated? Apical dominance is the phenomenon by which high (over the positive range limit) au

What is the importance of pantothenic acid, What is the Importance of panto...

What is the Importance of pantothenic acid One of the active forms of pantothenic acid is coenzyme A, which is involved in numerous metabolic functions. It is involved in t

How are the concepts of the dna, Q How are the concepts of the DNA, gene, a...

Q How are the concepts of the DNA, gene, and proteins characteristics of living beings related? Characteristics of organisms depend on chemical reactions that take place in the

What are the kinds of reproduction that occur in fungi, Q What are the kind...

Q What are the kinds of reproduction that occur in fungi? In fungi there is sexual and asexual reproduction. Fungi reproduce asexually by fragmentation, sporulation and gemmati

Alkoloids, what do you mean by alkoloids how are they usedin daily life wha...

what do you mean by alkoloids how are they usedin daily life what are the different types of alkoloids?

Taxonomy, . Explain why the traditional classification of unicellular euka...

. Explain why the traditional classification of unicellular eukaryotes as ‘protozoa’ or ‘protists’ is invalid in terms of modern systematics and evolutionary theory. Why are trad

Determine the working of law of diminishing return, Determine the working o...

Determine the working of Law of diminishing return According to the 'Law of diminishing return' the increase in the yield by a unit increase of the defi cient factor is proport

Explain about the effect of nutrient and drug interaction, Explain about th...

Explain about the Effect of Nutrient and Drug Interaction? Medicines can treat and cure many health problems. Nevertheless, do you recall your doctor advising or recommending c

Magnetic resonance imaging (mri), M a g n etic resonance imagin...

M a g n etic resonance imaging (MRI): It is in use as a clinical diagnostic tool since 1980. The distribution of hydrogen nuclei (protons), present in cellular wa

Argument, do you can write argument i will send you the article you want wr...

do you can write argument i will send you the article you want write from with rules please call me at 623-552-8532

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd