cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Metamorphosis, What type of metamorphosis do spongs go through is it reteog...

What type of metamorphosis do spongs go through is it reteogressive?

Relationship of ecology with other disciplines of biology, Relationship of ...

Relationship of Ecology with Other Disciplines of Biology In order to understand the scope and relevance of ecology let us consider its position in relation to other biological

How many grams of sodium dodecyl sulfate, How many grams of sodium dodecyl ...

How many grams of sodium dodecyl sulfate (mw = 288.37) would you dissolve in 80 mLs of water to make a 20% solution of SDS?

Biological diversity protection, Q. How can a great biological diversity pr...

Q. How can a great biological diversity protect an ecosystem from environmental damage? Why are less biodiverse ecosystems at risk of suffering deep biological harm if submitted to

Explain the consequences of malnutrition, Explain the Consequences of Malnu...

Explain the Consequences of Malnutrition? Malnutrition manifests itself in terms of illness and death in all age groups. Children, pregnant women, nursing mothers and elderly a

Nursing assessment of thalassemia patient, Nursing Assessment  If you ...

Nursing Assessment  If you observe a child of thalassemia major you can identify the following clinical  manifestations:  Anaemia with haemoglobin level of 3 to 8 gm per ce

Radiography, RADIOGRAPHY You  have read  in GNM courses about radiographi...

RADIOGRAPHY You  have read  in GNM courses about radiographical examination. In this text the discussion will be on the following radiographical tests.   Chest Roentgenogram

Explain pancreatitis, Explain Pancreatitis Pancreatitis :- Inflammati...

Explain Pancreatitis Pancreatitis :- Inflammation of the pancreas.

Phylum protozoa, what is the phylum protozoa and when he was discover

what is the phylum protozoa and when he was discover

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd