cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What do you know about cardiothoracic ratio, Q. What do you know about Card...

Q. What do you know about Cardiothoracic ratio? The cardiothoracic ratio (CTR) shown in figure is the transverse cardiac diameter (the horizontal distance between the most righ

What do you understand by taxonomy, Q. What do you understand by taxonomy? ...

Q. What do you understand by taxonomy? Taxonomy is dependent on many sciences and they in turn are equally dependent on it. The activities of a taxonomist are basic to all othe

State about the blood ocular barriers of the eye, State about the blood ocu...

State about the blood ocular barriers of the eye? Blood Ocular Barrier: All vascular beds of the eye are highly permeable to lipoid soluble substances (oxygen, carbon dio

Define nutrient requirements and principles of meal planning, Define nutrie...

Define nutrient requirements and principles of meal planning? In this , we started our discussion with the school age and its characteristics. We learnt about the physical grow

Cell theory, CEL L THEORY Proposed by Mathias Jacob Schleiden, 1838 (J...

CEL L THEORY Proposed by Mathias Jacob Schleiden, 1838 (Jerman Botanist), Theodore Schwann, 1839 (Jerman Zoologist) It consists of 5 fundamental facts - (i) Body of livi

Define the open loop system, Situation in which a closed loop negative feed...

Situation in which a closed loop negative feedback system is  converted to an open loop system?     A.  A disease that destroys all the adenylyl cyclase and all the cAMP in the

Health education to hospitalised child, Health Education to Hospitalised C...

Health Education to Hospitalised Child You have unique opportunity for teaching and reinforcing good hygienic care while the child is hospitalised. Clean hair, nails, clothin

Molecule synthesis and molecule break-down, Match dehydration reaction and ...

Match dehydration reaction and hydrolysis reaction to molecule synthesis and molecule break-down

Define the role of vitamin a in immune response, Define the Role of Vitamin...

Define the Role of Vitamin A in immune response? Vitamins A is essential to normal immune function and- regulation. As discussed above, during vitamin A deficiency the goblet c

What is the typical feature of the epithelia, Q. What is the typical featur...

Q. What is the typical feature of the epithelia? How different is it from the connective tissue? The typical feature of the epithelium is the almost absence or absence of space

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd