cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What could have caused the discrepancy, If the class's value did not match ...

If the class's value did not match the expected value of water, let's assume everyone was actually correctly using the devices and there was no "user error." What could have caused

What is drugs, What is drugs? Intravenous access must be established. A...

What is drugs? Intravenous access must be established. Although administration of drugs through a central vein is ideal in a low cardiac output situation, it is rarely possible

The best absorbed in an acidic environment, Why is a weekly acidic drug bes...

Why is a weekly acidic drug best absorbed in an acidic environment?

Development and differentiation of a flowering plant, Development and Diffe...

Development and Differentiation of a flowering plant The developmental phases of a flowering plant involve seed germination, vegetative growth, flowering, fruiting and senesce

Percussion - criteria for endosteal implants, Percussion - criteria for end...

Percussion - criteria for endosteal implants? It is neither an indicator of clinical health nor of rigid fixation. Ringing sound that occurs on percussion corresponds to the pr

Explain use of repellents, Use of repellents Avoidance and early remova...

Use of repellents Avoidance and early removal of ticks are the first steps in prevention of Lyme disease. In highly endemic areas, when an engorged Ixodes scapularis tick is at

Systematics, three types of evidence used by systematic taxonomists

three types of evidence used by systematic taxonomists

Accidents in industry workplace, Accidents in Industry Workplace It is...

Accidents in Industry Workplace It is a hard fact to bear in mind that accidents in industry workplace are to be avoided or discarded and efforts in this direction has been in

Principle for determination of blood glucose, Define Principle for Determin...

Define Principle for Determination of Blood Glucose by Nelson Somogyi Method? Proteins are precipitated from the sample and protein free filtrate is heated with alkaline copper

Explain full liquid diet, Explain Full Liquid Diet Full Liquid Diet:  ...

Explain Full Liquid Diet Full Liquid Diet:   This diet provides foods and fluids that are liquid or semi liquid at room temperature. The type of food provided may vary dependi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd