Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Bergmann's Method of Cell Plating
In this method free cells are suspended in a liquid medium at a density twice the finally desired plating density. Melted agar containing medium of otherwise the same composition as the liquid medium is maintained at 35?c in water bath. Equal volumes of the two media are mixed and rapidly spread out in petri dishes in such a manner that the cells are evenly distributed and fixed in a thin layer (about 1 mm thick) of the medium after it has cooled and solidified.
The dishes are sealed with parafilm. The cells to be followed are marked on the outside of the plate and before the colonies derived from individual cells grow large enough to merge with each other. They are transferred to separate plates. Another popular method for single cell culture is the microchamber technique, developed by Jones et al. (1960). In this method mechanically isolated single cells are cultured in separate droplets of liquid medium. While Jones et al. used sterile microslides and three coverglasses to make microchamber, it is now possible to buy pre-sterilised plastic plates with several microwells. Individual cells are cultured in separate wells each containing 0.25 ml of the liquid medium. The culture requirement of single cells increases with decrease in the plating cell density, and the cell cultured in complete isolation require a very complex culture medium. A simple medium conditioned by growing cell suspension for some time also fulfils the requirements of single cell culture at low density.
Issues Impacting Health and Development Poverty and malnutrition are the leading causes of high maternal and child mortality rates in developing countries. Together, they seri
karaka scientific name
Question 1 A patient is admitted to the hospital with suspected pulmonary tuberculosis. How would you confirm this disease? Answer the following questions a)
Define the Toxicity of Vitamin K? Vitamin K, does not produce any toxic effects in doses (10-20 mg) normally used for the treatment or subjects suffering from disorders of liv
What is the inactivation of the X chromosome? What is a Barr body? Inactivation of the X chromosome is a phenomenon that happens in women. As women have two X chromosomes only
Urine Collection: Untimed or random urine specimens are suitable only for few chemical tests (mainly qualitative test). Usually urine sample is collected over a predetermined
Determine the use of OPG An OPG is used to see the possibility for an anterior loop which is found to extend about 1-3 mm anterior to the mental foramen in about 12 per cent
Explain about the Lipoproteins? These are the Multicomponent complexes of lipids and proteins that form distinct molecular aggregates. They contain polar and neutral lipids, ch
Pathophysiology Leukocytes develop fiom stem cells located in the bone marrow. During leukocyte maturation, cells become committed to specific cell types and develop throu
excretory organs of protozoa
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd