Bergmann's method of cell plating, Biology

Assignment Help:

Bergmann's Method of Cell Plating

In this method free cells are suspended in a liquid medium at a density twice the finally desired plating density. Melted agar containing medium of otherwise the same composition as the liquid medium is maintained at 35?c in water bath. Equal volumes of the two media are mixed and rapidly spread out in petri dishes in such a manner that the cells are evenly distributed and fixed in a thin layer (about 1 mm thick) of the medium after it has cooled and solidified.

The dishes are sealed with parafilm. The cells to be followed are marked on the outside of the plate and before the colonies derived from individual cells grow large enough to merge with each other. They are transferred to separate plates. Another popular method for single cell culture is the microchamber technique, developed by Jones et al. (1960). In this method mechanically isolated single cells are cultured in separate droplets of liquid medium. While Jones et al. used sterile microslides and three coverglasses to make microchamber, it is now possible to buy pre-sterilised plastic plates with several microwells. Individual cells are cultured in separate wells each containing 0.25 ml of the liquid medium. The culture requirement of single cells increases with decrease in the plating cell density, and the cell cultured in complete isolation require a very complex culture medium. A simple medium conditioned by growing cell suspension for some time also fulfils the requirements of single cell culture at low density.


Related Discussions:- Bergmann's method of cell plating

Mechanism of urine formation, MECHANISM OF URINE FORMATION - Production...

MECHANISM OF URINE FORMATION - Production of urine is uripoisis or diuresis. First of all it was explained by Kushney. Nephron separates acidic urine from basic blood, so ma

Drug interactions, Amiodarone, quinidine, propafenone, and verapamil may in...

Amiodarone, quinidine, propafenone, and verapamil may increase digoxin levels up to 100 per cent. It is prudent to measure a blood level after 7-14 days (and at least 6 hours after

Define dietary goals in parkinson''s disease, Define Dietary Goals in Parki...

Define Dietary Goals in Parkinson's Disease? The main goals include: - Maintain desirable weight - Promote absorption of anti- Parkinson's drug levodopa - Lessen swall

Legal rights of psychiatric patients, LEGAL RIGHTS OF PSYCHIATRIC PATIENTS:...

LEGAL RIGHTS OF PSYCHIATRIC PATIENTS: When a patient is admitted in psychiatric hospital he may be deprived of  the freedom  to leave the hospital, and also to maintain certai

Explain phylum phaeophyta, Phylum Phaeophyta (brown algae) 1) They are ...

Phylum Phaeophyta (brown algae) 1) They are brown algae, apart from chlorophyll they have brown pigment i.e. fucoxanthin which predominates. 2) They are one of ,the most com

Describe st segment depression, Q. Describe ST Segment Depression? The ...

Q. Describe ST Segment Depression? The development of ST-segment depression with exercise is probably the most reliable sign if myocardial ischaemia and appears to be the most

Describe five different types of gateway vectors, Describe five different t...

Describe five different types of gateway vectors that are available, what different functions they can perform, and for what purpose. e.g.  (1) Vector pXXX, (2) function - expre

Induction in metamorphosis, Induction in Metamorphosis Some of the mo...

Induction in Metamorphosis Some of the morphogenetic changes throughout metamorphosis are found fo be quite independent of hormone action. For instance, generally during meta

Plant, what is comretun

what is comretun

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd