Basic principles of spectrophotometry, Physics

Assignment Help:

Basic principles of spectrophotometry:

An absorbance spectrophotometer is an instrument that measures the fraction of the incident light transmitted through a solution.  In other words, it is used to measure the amount of light that passes through a sample material and, by comparison to the initial intensity of light reaching the sample, they indirectly measure the amount of light absorbed by that sample.

Spectrophotometers are designed to transmit light of narrow wavelength ranges (see Figure 1 the electromagnetic spectrum).  A given compound will not absorb all wavelengths equally-that's why things are different colors (some compounds absorb only wavelengths outside of the visible light spectrum, and that's why there are colorless solutions like water).  Because different compounds absorb light at different wavelengths, a spectrophotometer can be used to distinguish compounds by analyzing the pattern of wavelengths absorbed by a given sample.  Additionally, the amount of light absorbed is directly proportional to the concentration of absorbing compounds in that sample, so a spectrophotometer can also be used to determine concentrations of compounds in solution. Finally, because particles in suspension will scatter light (thus preventing it from reaching the light detector), spectrophotometers may also be used to estimate the number of cells in suspension.

We will be using a spectrophotometer several times this semester to quantify the concentration of chemicals present in a solution.

 

922_Basic principles of spectrophotometry.png

 


Related Discussions:- Basic principles of spectrophotometry

Why michelson interferometer was designed, The Michelson interferometer was...

The Michelson interferometer was designed to study the nature of: a) water waves b) sound waves c) an "ether" d) sunlight Ans: The Michelson inte

Two elementary particles which have infinite life units, Name any two eleme...

Name any two elementary particles which have almost infinite life time? Electron and proton have almost infinite life time.

What is the colour of aluminium, Aluminium is (A) Silvery white in col...

Aluminium is (A) Silvery white in colour.   (B) Yellow in colour. (C) Reddish in colour.            (D) Pale yellow in colour. Ans: Aluminium is Silvery white in c

What do you understand by diffraction of waves, 1. What do you understand b...

1. What do you understand by diffraction of waves? Explain why the diffraction of sound is more easily observable than the diffraction of light? 2. The wavelength of two spectra

Determine the fringe width in youngs double slit, The fringe width in a You...

The fringe width in a Young's double slit arrangement is 0.008cm. What could be the fringe width if the slit separation is halved and the screen slit distance is doubled?

What is a neutral buoyancy, Q. What is a neutral buoyancy? Answer:- ...

Q. What is a neutral buoyancy? Answer:- Buoyancy is the net upward force knowledgeable by an object submersed in a fluid. Pascal's principle utters that fluid pressure on

Optics, A spider is hanging by means of its own silk thread directly above ...

A spider is hanging by means of its own silk thread directly above a transparent fixed sphere of r = 20cm .... the refractive index of material of sphere is root(2).... and height

Determine the grating element, A diffraction grating used at normal inciden...

A diffraction grating used at normal incidence provides a spectral line of certain order with wavelength 6000A 0 superimpose on another spectral line of wave length 5000A 0 of th

Kinematics, A particle has initial velocity 5 m/s due east and acceleration...

A particle has initial velocity 5 m/s due east and acceleration 2 m/s^2 due west. Find the distance traveled during 3rd second of the motion.

Pressure, a car tyre is pumped up to 230 kPa. What is the volume of air at ...

a car tyre is pumped up to 230 kPa. What is the volume of air at atmospheric pressure (100kPa) that must be pumped into a tyre that has a volume of 0.1 cubic meter?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd