Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Basic principles of spectrophotometry:
An absorbance spectrophotometer is an instrument that measures the fraction of the incident light transmitted through a solution. In other words, it is used to measure the amount of light that passes through a sample material and, by comparison to the initial intensity of light reaching the sample, they indirectly measure the amount of light absorbed by that sample.
Spectrophotometers are designed to transmit light of narrow wavelength ranges (see Figure 1 the electromagnetic spectrum). A given compound will not absorb all wavelengths equally-that's why things are different colors (some compounds absorb only wavelengths outside of the visible light spectrum, and that's why there are colorless solutions like water). Because different compounds absorb light at different wavelengths, a spectrophotometer can be used to distinguish compounds by analyzing the pattern of wavelengths absorbed by a given sample. Additionally, the amount of light absorbed is directly proportional to the concentration of absorbing compounds in that sample, so a spectrophotometer can also be used to determine concentrations of compounds in solution. Finally, because particles in suspension will scatter light (thus preventing it from reaching the light detector), spectrophotometers may also be used to estimate the number of cells in suspension.
We will be using a spectrophotometer several times this semester to quantify the concentration of chemicals present in a solution.
Water at 20°C flows through a long, straight pipe. The pressure drop is measured along a section of the pipe of length 1.3 m as a function of average velocity through the pipe. Res
Define, with the help of diagram, the principle and working of an a.c. Generator. Write the expression for the e.m.f. generated in the coil in terms of its speed of rotation
Maximum power transfer: Every source of EMF has internal resistance. If it is required to develop the maximum possible amount of power in an external load, then the load resis
Fermat's principle; principle of least time (P. de Fermat): The principle put by P. de Fermat, which states the path taken through a ray of light among any two points into a s
Role of Magnetic field in generating ultrasonic waves Magnetic field is applied parallel to the length of a ferromagnetic rod made of material as like iron or nickel, a small e
An aereplane has velocity 500m/s with respect to air. Wind blows with velocity 20m/s along north. Then in what direction pilot should be heading so as to go along North-east direct
What are the needs of light sources for fibre optic communication? Light sources for fibre optic communication should meet certain given requirements as illustrated below: a
Metallization: It is the process of giving electrical connections among different parts of the circuit. Aluminium is commonly used. It has a high electrical conductivity and a low
Fig- detremines a square loop Made from a uniform wire. Determine the magnetic field at the centre of the square if a battery is connected among the points A and C.
The total energy of a simple harmonic oscillator is 0.8 erg. What is its kinetic energy when it is midway between an extreme position and mean position?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd