Basic principles of spectrophotometry, Physics

Assignment Help:

Basic principles of spectrophotometry:

An absorbance spectrophotometer is an instrument that measures the fraction of the incident light transmitted through a solution.  In other words, it is used to measure the amount of light that passes through a sample material and, by comparison to the initial intensity of light reaching the sample, they indirectly measure the amount of light absorbed by that sample.

Spectrophotometers are designed to transmit light of narrow wavelength ranges (see Figure 1 the electromagnetic spectrum).  A given compound will not absorb all wavelengths equally-that's why things are different colors (some compounds absorb only wavelengths outside of the visible light spectrum, and that's why there are colorless solutions like water).  Because different compounds absorb light at different wavelengths, a spectrophotometer can be used to distinguish compounds by analyzing the pattern of wavelengths absorbed by a given sample.  Additionally, the amount of light absorbed is directly proportional to the concentration of absorbing compounds in that sample, so a spectrophotometer can also be used to determine concentrations of compounds in solution. Finally, because particles in suspension will scatter light (thus preventing it from reaching the light detector), spectrophotometers may also be used to estimate the number of cells in suspension.

We will be using a spectrophotometer several times this semester to quantify the concentration of chemicals present in a solution.

 

 


Related Discussions:- Basic principles of spectrophotometry

Gravitety, why fall an apple from a tree

why fall an apple from a tree

Explain concept of ideal gas, The ideal gas approximation: The given assump...

The ideal gas approximation: The given assumptions are suppose while defining an ideal gas: (i) The number of molecules in the gas is very huge. (ii) The volume V having the

Calculate the magnitude of the magnetic field , A 240-turn solenoid having ...

A 240-turn solenoid having a length of 21 cm and a diameter of 8.8 cm carries a current of 0.44A. Calculate the magnitude of the magnetic field inside the solenoid.

Theory to calculate magnetic moment of a bar magnet, Illustrate ‘tan A ‘and...

Illustrate ‘tan A ‘and ‘tan B' positions of a deflection magnetometer. Illustrate the theory to calculate magnetic moment of a bar magnet and Horizontal component of the earths

What is the condition for resonance, What is the condition for resonance? ...

What is the condition for resonance? Condition for resonance: The condition of frequency of the oscillatory circuit is equal to frequency of the vibrating rod as: 1/(2 Π

Work and energy, a person holds of hay over his head for 30 min and gets ti...

a person holds of hay over his head for 30 min and gets tired . has he done some wirk or not? justify your answer

Define an echo sound, An echo is easily the retransmission of speaker's ow...

An echo is easily the retransmission of speaker's own voice caused by reflection at a distance surface. If there is a sound reflector at a distance d from host, then the time in

Define ohm law, Define Ohm's Law Ohm's Law:  Relationship among the pot...

Define Ohm's Law Ohm's Law:  Relationship among the potential difference across an electric device and the current that runs by it.  V= I.R

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd