Bacteriophage, Biology

Assignment Help:

Bacteriophage is a virus which infects a bacterium and which is many times used in molecular genetics experiments as the vector, or cloning vehicle. Recombinant phages can be made in which certain non-essential l DNA is removed and then replaced with the DNA of interest. The phage can accommodate the DNA "insert" of about 15-20 kb. Replication of that virus will hence replicate the investigator's DNA. One would take use of phage l rather than a plasmid if the desired piece of DNA is rather large. Bacteriophage l and M13 are the ones usually used in cloning or subcloning of small genes or DNA fragments in E. coli. Bacteriophage P1 is one which is used for fragments up to 95 Kb in size. 


Related Discussions:- Bacteriophage

Describe the kinds of muscle tissues, Q. What are the kinds of muscle tissu...

Q. What are the kinds of muscle tissues? What are the morphological features that differentiate those types? There are three kinds of muscle tissue: the cardiac striated muscle

How does temperature affect the action of enzymes, How does temperature aff...

How does temperature affect the action of enzymes upon their substrates? There are explained temperature ranges under which enzymes operate and there is a specific temperature

Leucosolenia, special character of leucosolenia

special character of leucosolenia

Explain the s1m filament theory of muscle contraction, Explain the s1M fila...

Explain the s1M filament theory of muscle contraction. What is special of "FlavrSavr" variety of tomato? Why is it preferred to its normal native variety? Illustrate a label

What are fibroids, Fibroids, also called as leiomyomas, are benign smooth m...

Fibroids, also called as leiomyomas, are benign smooth muscle tumors that happen in the uterus. They grow under the influence of estrogen and progesterone, and are therefore seen i

Determine the term - neuropsychological approach, Determine the term - Neur...

Determine the term - Neuropsychological approach The neuropsychological approach relies on the use of tests in which poor performance may indicate either focal (localised) or d

What are the consequences of pem, What are the Consequences of PEM? Th...

What are the Consequences of PEM? The consequences of PEM are most often long lasting and irreversible. The common consequences include: • Irreversible growth retardation

Haemo filtration-open-heart surgery, Haemo Filtration : Ultra filtration d...

Haemo Filtration : Ultra filtration during open heart surgery helps in removing excess fluid, especially in renal failure patients. Patients are haemo diluted (haematocrit 18-20)

Food and diet, state one benefit of including vegetable fibre in the diet

state one benefit of including vegetable fibre in the diet

Construct cdna samples, Once you have identified a few relevant mRNA specie...

Once you have identified a few relevant mRNA species, you wish to more closely examine their messages by sequencing. You do not have the time to search the genome and isolate the D

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd