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Bacteriophage is a virus which infects a bacterium and which is many times used in molecular genetics experiments as the vector, or cloning vehicle. Recombinant phages can be made in which certain non-essential l DNA is removed and then replaced with the DNA of interest. The phage can accommodate the DNA "insert" of about 15-20 kb. Replication of that virus will hence replicate the investigator's DNA. One would take use of phage l rather than a plasmid if the desired piece of DNA is rather large. Bacteriophage l and M13 are the ones usually used in cloning or subcloning of small genes or DNA fragments in E. coli. Bacteriophage P1 is one which is used for fragments up to 95 Kb in size.
Describe the uses of pupil Variation in the size, shape or reaction usually indicates some form of disease, disorder or use of medication. The pupil is usually dilated by the u
Leishmaniasis Leishmaniasis is a group of disease caused by protozoa of the group Leishmania, and are transmitted to man by the bite of female sandfly (Phlebotomus). Three typ
Define Methods for determining pH? There are two general methods for determining pH of a solution. One is the colorimetric method and the other most common method is the use of
Senescence Plants begin their development after seed germination. They grow, flower and finally senesce and die. The period from the start to death is called the longevity or
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Explain the Bioavailability of Vitamin E? For dietary purposes, vitamin E activity is expressed as a-tocopherol equivalents (a-TEs). One α-TE is the activity of l mg α-tocophe
The A and B antigens in humans may be found in water-soluble form in secretions, including saliva, of some individuals (Se/Se and Se/se) but not in others (se/se). The population t
What is a cytoskeleton? What are its main constituents in animal cells? Cytoskeleton is the cytoplasmic structure that handles the cell, keeps its shape and fixates and moves t
Which statement is true of the energy levels of electrons in shells? A) All the electrons in an atom have similar amounts of energy. B) Electrons must lose energy to move from the
Meteorological Factors and Air Pollution Air pollution levels in a region are affected by wind, location, topography, precipitation and temperature inversions. Wind can carry
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