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Bacteriophage is a virus which infects a bacterium and which is many times used in molecular genetics experiments as the vector, or cloning vehicle. Recombinant phages can be made in which certain non-essential l DNA is removed and then replaced with the DNA of interest. The phage can accommodate the DNA "insert" of about 15-20 kb. Replication of that virus will hence replicate the investigator's DNA. One would take use of phage l rather than a plasmid if the desired piece of DNA is rather large. Bacteriophage l and M13 are the ones usually used in cloning or subcloning of small genes or DNA fragments in E. coli. Bacteriophage P1 is one which is used for fragments up to 95 Kb in size.
Indications 1) Symptomatic patient with normal LV function (ejection fraction 2 0.5 at rest). If they are in class In or IV NYHA, surgery is recommended. In this group of pat
Approaches to Neuropsychological Assessment Probably the most useful model of neuropsychological interpretation is described in Reitan and Wolfson's (1993) four approaches to a
Xenobiotic compounds are man-made chemicals that are present in the environment at unnaturally high concentrations. The xenobiotic compounds are either not produced naturally, o
Define the Term Epimerism? In addition to D and L isomerism sugars also exhibit epimerism. Hexoses that differ from each other as a result of variation of H and OH on carbons 2
Chloroplasts Like mitochondria, chloroplasts are also distinctive organelles of eucaryotic cell. But they are found only in plant cells. Chloroplasts contain membranes forming
What is L forms of bacteria?
a) The image above is part of a DNA sequencing gel. Assuming this is the DNA coding strand and you are reading 5' -> 3' what are ALL the possible polypeptides this sequence cou
List the different suturing techniques There are a various suturing techniques each suited for a particular situation. Few of the common suturing techniques are: i) Interrup
Removal of obturating material -Gutta percha . -Carrier bassed gutta perca. -silver points . - paste type. - obstructed fracture instrument .
A laboratory mouse homozygous for an RFLP marker is mated to a wild mouse that is heterozygous for that marker. One of the heterozygous individuals resulting from this cross is mat
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