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Bacteriophage is a virus which infects a bacterium and which is many times used in molecular genetics experiments as the vector, or cloning vehicle. Recombinant phages can be made in which certain non-essential l DNA is removed and then replaced with the DNA of interest. The phage can accommodate the DNA "insert" of about 15-20 kb. Replication of that virus will hence replicate the investigator's DNA. One would take use of phage l rather than a plasmid if the desired piece of DNA is rather large. Bacteriophage l and M13 are the ones usually used in cloning or subcloning of small genes or DNA fragments in E. coli. Bacteriophage P1 is one which is used for fragments up to 95 Kb in size.
Q. Explain Restrictive cardiomyopathy? It is a systemic or idiopathic disorder of the myocardium with clinical and hemodynamic features of diastolic dysfunction, closely simula
genetic reason.medications
Q. Why in bread and cake manufacture are alcoholic fermenting organisms used and not lactic fermenting organisms? Fermentation has the function of making breads and cakes grow
Define the Feeding and Nutritional Management of spinal trauma? The main objectives of nutritional management are to meet the nutritional needs of the initial acute phase and
Maintaining the quality of frying oil As frying continues, the level of oil in the fryer depletes. There are two beneficial frying fat quality factors affected during the fryi
Second Stage of Invagination The 2 nd stage of invagination starts with release of secondary mesenchyme from the tip of archenteron into the blastocoel. The cells of secondar
Colibacillosis Colibacillosis is caused by different strains of Escherichia coli. The disease mainly affects children and travelers. Organisms grow in gut and produce toxin. It ma
What is the difference between bryophytes and tracheophytes? Bryophytes are nonvascular plants (mosses, hornworts, liverworts), i.e., they do not have a conductive system for t
Q. Reduction in left ventricular? Vasodilators improve stroke volume, and reduce degree of regurgitation. This results from decrease in systemic vascular resistance and leads t
Ooplasmic Determinants and Somatic Cell Determination Eggs of tunicates go through typically mosaic development in which the various blastomeres become determined to follow th
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