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Bacteriophage is a virus which infects a bacterium and which is many times used in molecular genetics experiments as the vector, or cloning vehicle. Recombinant phages can be made in which certain non-essential l DNA is removed and then replaced with the DNA of interest. The phage can accommodate the DNA "insert" of about 15-20 kb. Replication of that virus will hence replicate the investigator's DNA. One would take use of phage l rather than a plasmid if the desired piece of DNA is rather large. Bacteriophage l and M13 are the ones usually used in cloning or subcloning of small genes or DNA fragments in E. coli. Bacteriophage P1 is one which is used for fragments up to 95 Kb in size.
Chemistry of Living Matter All living things including ourselves, are made of protoplasm. Protoplasm is essentiallya colloidal suspension of proteins in a w&ery solution. It is c
From your studies in ecology, you must be aware that most ecosystems are quite complex consisting of several habitats and that habitats in turn are composed of microhabitats. Each
Q. Extractive use of biodiversity? This includes direct use such as harvesting of wild plant species for use as food, fuel, fodder, fibre, shelter or medicine and hunting anima
Y-Linked traits in humans Any gene that occurs exclusively on the Y chromosome is said to be holandric and it is not expressed in females. Such a Y-linked gene normally occurs
What is the basis of classification of algae
CARDIO PULMONARY BYPASS : Open-heart surgery is considered as one of the most significant advances in medicine of 20th century. Establishment of safe cardio pulmonary bypass (CP
Gene or point mutations occur when the DNA sequence of a gene is altered and the new nucleotide sequence is passed on to the offspring. This occurs either due to addition or deleti
Air pollutants - Effects on plants Particulate matter such as cement, coal, petro coke, dust, and fly-ash screen the light and thus change its quantity and quality. Dust plug
The excretory organs and excretory materials of plants and animals
Contagious bovine pleuropneumonia (CBPP) This is a highly fatal disease of cattle and of major economic importance in certain tropical countries. It also affects buffaloes, bi
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