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Sudden addition of oxygen (O2) to a previously anaerobic culture of yeast fermenting grape juice results in a dramatic decrease in the rate of glucose consumption. This is known as "Pasteur effect". a) Why would the yeast cells consume less glucose in the presence of oxygen? Can you estimate how much less glucose the culture would use? b) Pasteur effect can be counteracted by the addition of 2,4-dinitrophenol (DNP), an uncoupler of oxidative phosphorylation. Why would DNP counteract or prevent the Pasteur effect?
A mammal uses only one to two percent of its energy in ventilation as a fish must spend about 15 percent of its energy to move water over its gills. Explain this huge difference in their efforts to collect oxygen.
In order to perform a fluorescence experiment, you need to seed a 4-well chamber slide with 60,000 cells in each well. To prepare for the cell count, you transferred twenty uL of cell suspension into thirty uL of trypan blue.
How many 10 micrometer yeast cells could fit across the field. In a guinea pig, black is dominant to brown, and solid colour is dominant to spotted. A heterozygous black, solid-coloured pig is mated with a brown, spotted pig.
Compare and contrast the gas exchange systems of fish and mammals in relation to their environments.
Membrane bound and free ribosomes, which are structurally and functionally identical, differ only in the proteins they happen to be making at a particular time.
What is the difference between octopuses and mussels regarding their circulatory systems? How does that difference influence the mobility of these animals?
An experienced goldfish breeder receives two unusual male goldfish. One is black rather than gold and other has a single fail fin rather than a split tail fin.
A store clerk tells you that vitamin C extracted from rose hips is better for you than synthetic vitamin C. Based on what you know of the structure of organic compounds, does this claim seem credible? Why or why not?
Distribute one cup with sugar water and one with fresh water. Take a sip of the sugar water and swish it around in your mouth for several seconds without swallowing it; gradually, it has to tasteless sweet. After swallowing it (or spitting it back in..
Assume you are conducting a mark-recapture study of a population of snouters. During your first sampling period you go out and catch or mark or release 141 snouters.
You do DNA analysis of a newly found species of mammal and find there to be 7 JK and 65 VK sequences. Assuming that any V sequence can join any J sequence, how many different kappa light chains can this mammal make?
Estimate which of the following classifications of the epithelia is key for normal implantation of the zygote?
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