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A)In a classic experiment, cells were treated with tritiated thymidine and fixed immediately. The cells were then treated for autoradiography and then examined in a microscope. What the researchers were looking for was the timing of DNA synthesis. First, why use tritiated thymidine and not some other nucleotide?
B)In this experiment described above, the researchers looked specifically for mitotic figures, that is cells actively undergoing mitosis. They saw no radioactive mitotic figures. Why not?
C)How would you do the experiment described differently so that labeled mitotic figures would be observed?
Explain the events that occur when epinephrine binds to a G-protein coupled receptor in the membrane of a cell. Begin with the binding of the epinephrine and end with the activation of adenylyl cyclase.
In dogs, dark coat (D) is dominant over albino (d) and short hair (S) is dominant over long hair (s). After crossing dogs with dark and short hair with albino dogs with long hair, in F2 a breeder got 89 dogs with dark and short hair, 31 dark, long..
The Fas ligand is a trimeric, extracellular protein that binds to its receptor, Fas. The binding of Fas ligand to its receptor alters the conformation of Fas so that it binds an adaptor protein and activates caspase-8.
A 20-year old man presents with pain and swelling about the left knee for the past month. He thought that this condition resulted from an old football injury and that it would resolve without incedent. The pain however has persisted and is severe ..
what are four natural occuring enzymes used in Recombinant DNA technology, and describe their natural and technological use.
Discuss the greatest effect on determining which mutations will go to fixation in both a large population and a small population?
If all students start with the same amount of 10,000 x g supernatant, and after different groups of students have done their 40-65 percent ammonium sulfate cut.
How do you explain why enhancers can be moved around within the DNA without affecting their activity, whereas the TATA box can only operate at one specific site?
Considered the simple cell from above (with initial volume 3nL containing .3 M protein and .2M Ca++). This cell is immersed in a 0.2M uera bath.
Explain: (a) why we use scientific names written in Latin (and sometimes Greek) and (b) the importance of binomial nomenclature in naming organisms. Give examples of three scientific names (in correct binomial format) of common organisms.
Assume you ask is to work with the genetic code and examine the structure and characteristics of the amino acids used to make proteins and answer the following question.
The molecules of all matter, particularly gases and liquids, are in constant vibration motion. As a result of this motion, molecules are continually colliding with one another, much like balls scattering over a pool table.how to Add a coverslip ov..
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