Why might an extreme buffer ph 6 to 9 be unsuitable

Reference no: EM137362

Q1. A small, impermeable membrane is placed between the anchor cell and the other vulva precursor cells in a larva of C. elegans. What would you expect the result to be?

Q2. Your first project in your new lab is to purify the protein Rock Band (RB) which is known to be phosphorylated by the kinase Heavy Metal Drummer (HMD). As the new kid on the block, you are tasked with purifying tons of HMD for the lab to use. You first attempt to purify the kinase using ion-exchange chromatography. You scour PubMed and determine that the isoelectric point (pI) of HMD is pH 5.5.

(i) What have to the pH of your buffer been if you wanted to bind HMD to an anionexchange column and why?

(ii) What should the pH of your buffer be if you want to bind HMD to a cation-exchange column and why?

(iii) What potential issues might arise if the pH of your buffer is too close to the pI of your protein of interest? Why might an extreme buffer pH (< 6 or > 9) be unsuitable?"

Reference no: EM137362

Questions Cloud

Why is the term hominid cladistically indefensible : The term "hominid" has traditionally been used to refer to species in the line the leads to modern humans after the divergence between that line and the line that leads to modern chimpanzees.

What is biological psychology : The small intestine is specialized for absorption by the huge number of villi that line the intestinal wall. Villi have cells that generate intestinal enzymes which complete the digestion of peptides and sugars.

Utilize the index to identify the disease : Explain physiological changes that occur in three different organ systems when food is ingested and how these systems coordinated a response in the human body.

What are the limiting factors of ecosystem : Explain the plant life, animal life, and geology of the ecosystem in the area in which you live. What populations and communities are there? How dependent is your community on this ecosystem? What are the limiting factors of ecosystem.

Why might an extreme buffer ph 6 to 9 be unsuitable : A small, impermeable membrane is placed between the anchor cell and the other vulva precursor cells in a larva of C. elegans. What would you expect the result to be.

Allow animals and plants to migrate between refuges : In many countries, conservationists are trying to design national park systems so that "islands" of natural area (the big parks) are connected by thin "corridors" of undisturbed habitat. The idea is that this arrangement will allow animals and plants..

Oxidize sugars to yield useable cellular energy : Recognize precisely which areas of the living human brain are most active in participants who are trying to solve a mathematical problem.

What fraction of total dna the target sequence constitute : The genome of virus SV40 is a circular double-stranded DNA molecule 5 kb long. A 1 kb region is amplified by PCR for four cycles. What fraction of total DNA does the target sequence constitute.

How genetic material is replicated : Eukaryotic cells are more complex than prokaryotic cells. Explain their differences, functions and the composition of each including how genetic material is replicated.

User Account

All Pages