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Why is the axon unable to generate an action potential

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If the seawater bathing the squid giant axon is replaced with artificial seawater in which NaCl is replaced with choline choride (which does not cross the membrane), there is no effect on membrane potential, but the axon no longer generates an action potential when stimulated. Why is there no effect on membrane potential, and why is the axon unable to generate an action potential?

Reference no: EM13139359

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