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Why is it important to have an antibiotic resistance gene in the cloning plasmid? a. It makes the bacteria resistant to the antibiotic. b. It makes the bacteria produce more clones. c. It provides a way for sorting bacteria that have the gene of interest from the bacteria that just have an "empty" cloning plasmid. d. It provides a way for researchers to sort the bacteria that have the cloning plasmid from the bacteria that don't have the cloning plasmid. e. It aids scientists in developing new antibiotic treatments.
List the seven major biomes discussed in the text and give a brief description of the climax vegetation in each.
Explain a muscle contraction beginning with the motor neuron to relaxation of the muscle. Discuss the physiological relationship of the muscular and skeletal system.
Which statement about night blindness is most accurate.
Suppose that you measure the OD of two E.coli cultures: culture A and culture B. The spectrophotometer gives you a reading of 2.2 for culture A and 0.02 for culture B.
discuss the effectiveness of each antibiotic on each type of bacteria keep in mind that antibiotics may or may not be specific to certain types of organisms?
Fungi are the primary decomposers in ecosystems. If ecosystem lacked fungi, what impacts would that have on plant and animal nutrition?
E. coli were transformed with 50 ng of plasmid DNA is a total volume of 500 µl, and 100 µl of the transformation reaction was plated on a LB amp agar plate. Count the colonies and calculate the transformation efficiency for this experiment.
During membrane filter method, for testing fecal contamination in drinking water how would grit on the filter paper affect your confidence in your data results? How should the method for determining coliform density be altered?
Determine the coefficient of coincidence and the level of interference. What do theses two terms mean?
What is Mrs. X red blood cell volume after the infusion equilibrates with the blood and why? Also what is the osmolarity of the red blood cells and plasma AFTER equilibration.
In a protein sequence, which repeated sequence over a twenty amino acid stretch would most likely lead to insertion of a transmembrane domain into a lipid bilayer.
describe the main causes of pituitary dysfunction and the methods used to evaluate normal/ abnormal hormone secretion- hormonal levels, feedback control and 'pituitary function' tests used in the clinic.
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