Why is it important that the bacteria are in log phase

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Reference no: EM134041186

Assignment:

Lab: Bacteriophage and Determination of PFU

1. Why is it important that the bacteria are in log phase? In log-phase growth, it creates a dense, turbid layer of bacteria able to sustain viral growth.

2. What is the control?

3. What do you expect the control plate to look like and why?

4. What is the ideal plaque count range?

5. Which dilution(s) yielded results in the ideal range

6. You have performed a serial dilution of phage, incubated 100 l of the phage dilution with E. coli below is your plaque count. Using the formula from the question above, determine the PFU for each dilution and the concentration of the stock solution. Plaque count Viral dilution PFU/ml 950 10-4 11 10-6 100 10-5

7. What are 3 differences between a bacteriophage and a bacterium?

8. How could you easily determine in the laboratory if the phage was lytic or lysogenic?

9. What is a serial dilution? Draw/describe a serial dilution of 625-fold from a stock viral solution. You will perform the dilution in 4 steps. The total volume of each tube in the series is 500 l. *Hint: look in the lab manual to review dilutions.

What is your dilution factor? 1:____?

Volume of diluent:_____?

Volume of virus: ______?

How might phage be used as a treatment for a bacterial infection?

Part II-Phage Transduction

Watch the phage transduction video and answer the following questions:

Do phage particles enter the cell during viral replication?
How does transduction contribute to bacterial genetic diversity?
Why do phage package host DNA?
How do you select for transduction?

Reference no: EM134041186

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