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1. Explain your evidence for coming to this conclusion. Is this sufficient evidence to convict the suspect of the crime? Why or why not?
2. It what way did this lab simulation differ from what is actually performed in a forensic analysis?
3. Why is restriction fragment length polymorphism (RFLP) commonly refered to as a "DNA fingerprint"?
4. A restriction endonuclease "cuts" two DNA molecules at the same location. What can you assume is identical about the molecules at that location?
5. Do any of your suspect samples appear to have EcoRI or Pstl recognition sites at the same location as the DNA from the crime scene? If so which suspects and what are the band lengths (bp) that match?
6. Why is DNA sometimes refered to as a palindrome?
If you need to make 1 lite of a 1x solution of western transfer buffer, how would you prepare it from a 10x stock?
Using light microscopy you examine the soil samples and the goo from the degraded polyurethane. Will this approach allow you to observe all microorganisms present in the sample ? why or why not ? What are the limitations of this approach?
Assume you start with 10 ml of 2% acetic acid, and you add 90 ml of water. We will call the resulting solution "dilution#1". What is its concentration?
Describe how a linear sequence of amino acids gives rise to the three-dimensional structure of proteins. Detail the significance of this structure, how it may be modified, and the results of such modification.
For a new mutation arising in the XYZ gene designated XYZG287 there is a linked allele, QRS2 ~100,000bp away on the same chromosome. After twenty generations find the estimated linkage disequilibrium.
Stenohaline osmoconformers such as sea anemones and sea stars, pH of arterial blood is slightly higher than that of venous blood because
You identify a novel bacterial species from a deep sea hyrothermal vent that lacks 5'-dNTPs. The DNA in this organism has the normal anti-parallel double stranded helix with 3'- 5' phosphodiester bonds.
How will chronic Hepatitis C impact a female's reproductive system? Discuss sex hormone levels, hypothalamic-pituitary axis changes, fertility and menstruation changes.
On large, black lava flows in the deserts of the southwestern U.S., populations of many types of animals are composed primarily of black individuals. By contrast, on small lava flows, populations often have a relatively high proportion of light-co..
What would be the biological advantage for organisms/cells that have alternative energy pathways such as fermentation?
In plant communities today, leaf morphology is correlated with mean annual temperature, so paleobotanists use fossil leaf morphology to estimate the mean annual temperature of paleoclimates.
A microbiologist found a new restriction endonuclease. When DNA is digested by this enzyme, fragments that average 1,048,500 bp in length are produced.
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