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1. Why does Synechocystis not require electroporation or chemical pretreatment prior to transformation?2. What specific genetic manipulation does Synechocystis readily allow that E. coli does not?3. For transformation of Synechocystis, why do you first plate your transformant mixture on a filter on a BG - 11 plate with glucose, and the next day the TA transfers the filter with cells to a plate that contains kanamycin (rather than plating directly on kanamycin)?4. What is the product of the psbC gene? What is its function?5. In the PCR reaction, what is the role of each of the PCR reagents: primers, dNTPs, Mg2+, and Taq polymerase?6. What are the considerations in determining the number of cycles needed for the PCR reaction?
In an enzyme catalysed reaction, if the activation energy of the transition state is decreased, which of the following will occur?
The following cross involving three genes was performed: Aa Bb Cc x aa bb cc, and the phenotypes and number of progeny are shown below. Each pair of alleles exhibits simple dominance and recessiveness.
A study is planned to compare two weight loss programs in patients who are obese. The 1st program is based on restricted caloric intake and the second is based on specific food combinations.
Small molecules move through membranes in different ways -- different for different molecules or compounds, that is.
suppose a bird that eats grasshoppers moves into the prairie. How will this affect natural selection of the grasshoppers? How might this change in a drought year.
a bacterium will construct different proteins to metabolize the sugars lactose or glucose, depending on which one it detects in the outside environment. What part of the bacterium allows it to recognize different substances in the outside environm..
Are temperature gradients present in all biomes? What is one example of an adaptation to a colder temperature range?
Define stable equilibrium in population genetics. Describe the kind of data that you would need to collect about a population to conclude that it is in a stable equilibrium.
Given that the average adult consumes 2000 calories per day. There is slightly less than 4 dietary calories (Kcal) in a gram of sucrose (table sugar). One mole of sucrose weighs 342.3 grams. 1mol=6.02*10^23 molecules. Dietary fats (typically 18-20..
Assume you have one hundred ml each of 400 mg/ml sodium phosphate, 250 mg/ml sodium chloride, and 100 mg/ml potassium nitrate.
How does bioremediation control levels of pollution?
A certain cell, which is surrounded of an extracellular, hypertonic environment, needs to move a molecule of sucrose out of the cell. What type of transport mechanism would it use? (Only choose one)
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