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Unlike microscopy, cell fractionation and protein purification is often performed as a series of linked steps that can often take several days or longer than a week's time.
a. Most buffer solutions used in this process are isotonic and contain protease inhibitors. Why would this be the case?
b. EGTA and some types of protease are commonly used for cell dissociation. Why are each important and why do some cells dissociate from each other more easily than others?
While Mexican hairless dogs are crossed with normally haired dogs, about half the pups are hairless and half have hair. While, though, two Mexican hairless dogs are mated, about a third of the pups produced hair, about two thirds are hairless and som..
Suppose if life originated once on Earth, we might expect that it should be able to originate again, much like Lamarck hypothesized, but we do not observe this happening.
A dihybrid cross of two plants is done and the seed pod shape is examined
Describe why topoisomerases are required in eucaryotic cells even though eucaryotic DNA molecules are linear, not circular like procaryotic DNA molecules.
the oxaloacetate-malate couple is less negative than the NAD_- NADH couple. How are these values compatible with the transfer of electrons from malate to NAD_ in the TCA cycle?
E. coli were transformed with 50 ng of plasmid DNA is a total volume of 500 µl, and 100 µl of the transformation reaction was plated on a LB amp agar plate. After overnight incubation at 37C.
What is the relationship between an increase in light intensity and photosynthetic rate in leaves from a corn plant? How does this association compare with what you observed for tomato plants.
Select an organism which consists of a population having two distinct traits. You can be creative here (red and green beetles, humans with red hair and brown hair, birds with short beaks and long beaks and so on).
A certain cell, which is surrounded of an extracellular, hypertonic environment, needs to move a molecule of sucrose out of the cell. What type of transport mechanism would it use? (Only choose one)
It is January and the starting of a new semester. Just as you get settled in to your new routine, you begin to notice that illness is spreading across campus and other students in your classes are so sick they do not come to virology class.
Suppose you poured iodine on your plate and notesed clearing in the uninoculated area, as well as around both of your transferred cultures. What are some possible explanations for this occurrence?
The given sequence of a piece of mRNA. Create the sequence of the double stranded piece of DNA from which it was derived. Label the strands: coding, noncoding, template, nontemplate.
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