Why different plasmids isolated after the ligation reaction

Assignment Help Biology
Reference no: EM13158803

Explain any possible reasons why different plasmids isolated after the ligation reaction might contain recombinant pieces with different single base changes even though they are all of identical length.
(Possible hints are the choice of polymerase and the complexity of the genomic DNA.)

Reference no: EM13158803

Questions Cloud

Define the mass of hydrogen that combines : Hydrazine, ammonia, and hydrogen azide all contain only nitrogen and hydrogen. The mass of hydrogen that combines with 1.00g of nitrogen for each compound is .144, .216, and .240, respectively.
Six major steps involved in infectious disease : Explain the six major steps involved in infectious disease prevention planning at the community level.
Why there is difference in the number of amino acids : The Staphylococcus aureus Nuclease A protein produced on the ribosomes consists of approximately 209 amino acids. However the secreted version which is active extracellularly consists of only 149 amino acids. Explain why there is difference in the..
Secant method to tryt to find out the root : create a function that will use the secant method to tryt to find out the root
Why different plasmids isolated after the ligation reaction : Explain any possible reasons why different plasmids isolated after the ligation reaction might contain recombinant pieces with different single base changes even though they are all of identical length.
The ocl- form of this acid is less effective : What is the resulting pH of this solution? b. The OCl- form of this acid is less effective as a disinfectant
Determine the boiling point of a solution : Determine the boiling point of a solution of 22.5 g of fructose, C6H12O6 , in 294 g of phenol.
Determine the cash inflows and outflows : Messier company is planning to finance several projects and wants you to determine the cash inflows and outflows of the following bonds. (the market interest rate for Messier company is 8 percent.
Design a function that accepts an integer : Design a function that accepts an integer argument and returns the sum of all the integers from 1 up to the number passed as an argument. For example, if 50 is passed as an argument, the function will return the sum of 1, 2, 3, 4, . . . 50. Use recur..

Reviews

Write a Review

Biology Questions & Answers

  How are chromosomes rearranged in deficiencies

How are chromosomes rearranged in deficiencies, duplications, paracentric inversions, pericentric inversions, and reciprocal translocations?

  Removing 1000 cubic kilometers per year from rivers

There is approximately 2,000 cubic kilometers of water stored in rivers around the world. Using the terms water cycle, flux and pool, explain under what situations removing 1000 cubic kilometers per year from rivers for human use could be sustainable..

  Compare and contrast genetic drift and inbreeding

Compare and contrast genetic drift and inbreeding in each of the following areas: When acting alone, how do they each affect heterozygosity.

  What is wrong with monica

Monica begins typing her term paper on her computer early one morning and after 8 hours of typing, she notices that her wrists are stiff and very sore. The next morning she begins to finish the paper, but soon finds her wrists hurt more than last ..

  Assume that the na+ ions are moving from outside

Calculate the maximum [glucose]in/[glucose]out ratio that can be achieved by the plasma membrane Na+-Glucose symporter of an epithelial cell when the concentration of Na+ inside the cell is 12 mM.

  How many cells does the average human have

Identify the different phases and stages of the mitotic cell cycle.Explain the mitigating factors for transition from one phase and stage to the next phase and stage?

  What are some biotic factors involved in the nitrogen cycle

What are some biotic factors involved in the nitrogen cycle? Are there any abiotic factors to be considered?

  Which is preformed when blood pressure or heart rate is high

The baroreceptor reflex can be used to our benefit in a procedure known as a carotid sinus massage , which is preformed when the blood pressure or heart rate is pathologically high. Why would a massage of the carotid sinus help treat these conditi..

  Explore the role of the epigenome

Explore the role of the epigenome and/or other gene expression regulatory mechanisms in the development of cancer.  Discuss in detail one possible epigenetic/post-transcriptional/post-translational target for cancer treatment, and identify the succes..

  Question about olfactory receptor gene

Each olfactory receptor gene is involved in detecting specific odors. However, humans are losing OR genes, while mice and other mammals have ~1500 such genes, humans have ~900

  Describing the modern eating habits

Discuss the differences you have noticed between old and new Food Pyramid. Provide your opinion if you believe new Food Pyramid will be successful in modifying the eating habits of Americans or not.

  Which of the secondary sex characteristics in females

Which of the following are secondary sex characteristics in females?

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd