Why bioprocess engineers may want to genetically modify

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Reference no: EM132391515 , Length: word count : 1000

Answer these five questions in no more than 200 words each.

The goal of these questions is to demonstrate your understanding of the course material. Feel free to put your replies in context by citing sources of information or examples other than the textbook.

Avoid verbatim copy of the textbook or web resources. Plagiarism will be verified using software tool and will lead to 0 point for the corresponding question.

Question 1: Discuss why bioprocess engineers may want to genetically modify a living organism.

Question 2: Discuss the respective merits and limitations of batch, fed-batch, and continuous fermentation.

Question 3: Discuss the benefits and issues associated with the immobilization of enzymes involved in a biochemical process

Question 4: Discuss the issues associated with the scale up of a biological process.

Question 5: You are a project manager in a contract manufacturing organization. A client gives you a bacterial strain that expresses an enzyme that the client wants you to produce at a large scale (grams). The client also gives you a validated protocol to purify the protein. Denaturing gel electrophoresis shows that the enzyme is abundantly expressed by the bacteria you received from the client. However, your team cannot detect any enzymatic activity after applying the validated purification protocol to the biomass. How do you interpret this paradox and what can you do to increase the yield of your process?

Reference no: EM132391515

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