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A type of phosphatase can be assayed using p-nitrophenyl phosphate but the assay is performed at pH 7.8, which is the optimum for this enzyme. The initial velocity measured at the highest substrate concentration was .45 deltaA_400 / minute. Convert this velocity to a change in concentration/minute (microM/minute). HINT Use the pKa of p-nitrophenyl (7.0) and the henderson hasselbalch equation to correct the concentration, since at pH 7.8 not all of the product is in the colored, visible form. Use 18,380 M^-1cm^-1 for the extinction coefficient.
Illustrate one experiment that shows that shh is the critical molecule produced by the inducing tissue referred to in (a). what is the evidence that this tissue regulates the ventral pattern of the neural tube (even before this tissue was known to pr..
Question: A patient in your care has a total body water volume of 42 Liters, a hematocrit of 40 percent an extra celllular fluid volume of thirteen liters and a plasma osmolarity of 300 mOsm/ liter.
In agrobacterium transformation, after infecting leaf discs with agrobacterium, they are incubated on the co-cultivation plates and transferred to the callus-inducting media.
Suppose the Sun had a temperature of 17,400 K (three times its present temperature) but was the same size it is now. How much more energy would the Sun emit per second.
Each lymphocyte in circulation changes over the life of the cell and develops certaincharacteristics, particularly after an encounter with an antigen. Determine which organic macromolecules are most antigenic?
After taking samples of HIV RNA sequences from two infected patients, you plot nucleotide differences over 500 generations. You calculate a y-intercept of -5 and a slope of 0.02.
Determine the other components of the master mix besides nucleotides and what are their functions?
What is the rate of coagulation of naturally occurring colloids (under various pH and I scenarios)? How may the size spectrum of agglomerates change over time? How quickly do colloidal aggregates settle.
The sickle cell allele remains quite high populations of those with African Ancestry, and worldwide distribution of the sickle cell gene matches very closely to the worldwide distribution of Malaria.
he p K 1 , p K 2 , and pK R of the amino acid lysine are 2.2, 9.1, and 10.5, respectively. The p K 1 , p K 2 , and pK R of the amino acid arginine are 1.8, 9.0, and 12.5, respectively
List and express at least three prokaryotic cellular features that might be targeted in this way to inhibit or kill a bacterial pathogen.
Discuss the difference between a totipotent cell and a differentiated cell, genetically and morphologically?
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