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This question concerns three E. coli genes. Genes a and b eachencode polypeptides of 300 amino acids, while gene cencodes a polypeptide of 3,000 amino acids.
In trying to isolatemutations for each of these genes, I treated cells with a chemicalto introduce point mutations into the DNA; using this method anynucleotide in the genome has an equal probability of being mutated.I then screened for mutations in each gene. I found that one inevery 10,000 cells carried a mutation in gene a. Thus themutation frequency for gene a is 1 in 10,000.
a. Given this information,what would you estimate to be the mutation frequency for geneb?
b. What is the probability that a single bacterial cell would carry mutations inboth gene a and gene b?
c. What would be the likely mutation frequency for gene c?
Create a point mutation in the gene that will result in a genotype change, but not a phenotype change. Circle the point mutation on the DNA strand and name an agent that causes point mutations.
Design an enrichment medium to isolate a detergent-degrading bacterium that is found in soil.
The hydrolysis of pyrophosphate to orthophosphate is in driving forward biosynthetic reactions, such as the synthesis of DNA. This hydrolytic reaction is catalyzed in E.
Investigate a disease that is caused by a chromosomal mutation. What is the type of mutation? What chromosome is affected? What are the consequences? (Minimum of 250 words)
Why does meat contain DNA?
When some individuals are startled they hyperventilate. Why is this occurring? List the vessels in order of ascending pressure within the circulatory system
What is the difference between theory and hypothesis and explain the importance for a hypothesis to be testable and falsifiable in order for the scientific method to be applied
your local water district proposed a ban on household triclosan-containing products. Explain the rationale for such a ban?
What is a good Hypothesis for What's my problem? Is it ADD or is it ADT?
Often right parietal damage cause hemispatial neglect. Describe 2 deficits that may be displayed.
If a transferrin receptor has a cross-sectional area of 50 Angstroms X 100 angstroms (5x10nm), and how many receptors could be accomodated in a 100nm clathrin-coated vesicle assume that only 50% of surface area can be occupied by proteins.
Compute the surface-area-to-volume ratio of a cube whose sides are 100 um in length to estimated the surface-area-to-volume ratio of the fern spore cell.
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