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Assume that a culture of E. coli was grown for approximately 50 generations in 15N (provided in the medium in the ammonium ion), which is a heavy isotope of nitrogen (14N). You extract the DNA from the culture, and it has a density of 1.723 gm/cm3 (water = 1.00 gm/cm3). From the literature, you determine that DNA containing only the common form of nitrogen, 14N, has a density of 1.700 gm/cm3. Bacteria from the 15N culture were washed in buffer and transferred to 14N medium for one generation immediately after which the DNA was extracted and its density determined.
What would be the expected density of the extracted DNA?What band(s) would be expected if subjected to ultracentrifugation through a CsCl gradient?(b) How would your answer to (a) above differ if replication occurred in a conservative fashion?(c) Assume that the extracted DNA was completely denatured (separated into two single strands by heating at 90oC for 15 minutes), what would be the density (or densities) of the DNA (s) in the heated extract?
Niremberg used the enzyme polynuclotide phosphorylase to assemble RNA with random sequences in the absence of a template. Suppose they added the enzyme to a solution of 90% GTP and 10% UTP, and then used the cell-free system of protein synthesis t..
Critically explain the environment and outcome of the environmental problems, the strategies and factors which affect the environment and prevention of the environmental strategies with the graphical explanation.
Nocodazole reversibly inhibits microtubule polymerization, which is essential for formation of the mitotic spindle. By treating a population of mammalian cells with nocodazole for a time and then washing it out of the medium.
A white albino mouse was crossed with a wild grey colored one. The first generation mice were all grey. In the second generation, there were 198 grey and 72 albino mice.
Determine the percentage of offspring displaying each trait. If a single, diploid G2 nucleus in an ascus contains 400 nanograms (ng) of DNA, after that a single ascospore nucleus of this species should contain how much DNA (ng), carried on how many c..
Identify the elements and the number of atoms of each element in each of the following compounds.
Assume if a eugenic sterilization law were universally enforced, such that all feebleminded individuals were sterilized before reaching sexual maturity.
What percent of all the enzyme molecules will have their Cys thiol in the active form at pH 8.6. Some mammals might run or fight when frightened. For both of these examples, illustrate the physiological mechanisms involved in the answer.
In the stable form of protein, what is generally oriented to the interior of the protein molecule?
How does divergence effect Fst values between 2 populations? AND List 3 reasons that the Ne will be smaller than N. In each case, explain HOW this scenario results in a smaller Ne. Detailed answers get the points.
What are its symptoms and how can it can be diagnosed? How can the infection or disease be prevented? What makes this infection or disease different from others you have studied?
Molly requires to make one liter of a 1.5 M glucose solution for use in an experiment. The molar mass of glucose is 180.16 g/mol.
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