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In a mutant strain of E. coli, a tRNA that recognizes the codon 5' CUG 3' of normal cells has a point mutation so that it now recognizes the codon 5' GUG 3' while still carrying its original amino acid. Surprisingly, a missense mutation found in a protein-coding gene is no longer causing problems for the cell due to the presence of this mutant tRNA.
a. What amino acid is this tRNA carrying?
b. Before the missense mutation in the protein-coding gene, what was the original encoded amino acid?
c. Before the change in the tRNA gene, what missense amino acid was incorporated in protein?
Early in development, most human cells turn off expression of essential component of telomerase, enzyme responsible for addition of telomere repeat sequences (5' TTAGGG 3') to the ends of chromosomes.
What is wobble, and how does it affect the number of tRNAs needed to translate the codons of a particular amino acid?
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what osmotic problems does a plant living in salty soil have? Explain and what osmotic problems might a fresh water fish have? explain.
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