What other sequencing technology would you use

Assignment Help Biology

Reference no: EM133434444

LGM is a newly discovered alien species on Mars. LGM individuals have two feet and two arms and are humanlike. The notable differences are that they have three eyes and three fingers. LGMs resemble the Squeeze Toy Aliens from Toy Story movies but are less cute.

As a sole exobiologist on the Mars Mission I, you are interested in understanding the biology of LGMs. You have brought all major sequencing and biotechnological instruments to the Mission. From your BIOL5381 class at UTD, you remember that getting the genomic sequence would be the first step for studying these aliens. You obtained a blood sample of one LGM volunteer and purified DNA from its blood. You decide to perform shotgun sequencing of the LGM genome using Illumina sequencers and obtain millions of 200 basepair long sequences. You are able to assemble these fragments into 7 long contiguous segments (contigs) that you label A, B, C, D, E, F, and G. From the sequencing experiment; you also recovered many 200 basepairs fragments that align as an alpha satellite repeat that is several kilobasepairs long. Still, you weren't able to place this repeat confidently with other contigs.
A and B resemble each other and are highly similar in sequence. C and D resemble each other and are highly similar in sequence. E, F, and G resemble each other and are highly similar in sequence. You assign these sequences as potential chromosomes: A and B as chromosome 1;
C and D as chromosome 2; and E, F, and G as chromosome 3 (Figure 2). The initial assignment gave the chromosome number of 3. Chromosome 3 has a point centromere that was easily identified from the sequence. Telomeric DNA was found on both ends of chromosome 3. No centromeric DNA was found on chromosomes 1 and 2. Chromosomes 1 and 2 also have only one telomere at only one of the two ends and are missing the telomeric sequence at the other end.

Question 1. Based on the information above, what might be the actual (or true) number of chromosomes for LGM individuals sequenced?

Question 2. What other sequencing technology would you use to verify the observed or hypothesized number of chromosomes for the LGM individual? Why?
Upon examining the basic features of many thousands of LGMs, you identify several LGMs with only 2 fingers on one or both hands (on the left hand only, on the right hand only, or on both hands). Using a karyotyping method, you determine that these rare LGM individuals with only two fingers have only two chromosome 3 instead of three normally found.

Question 3. Why would missing one chromosome 3 lead to the two-finger phenotype?

Question 4. Why isn't the two-finger phenotype the same among these LGM individuals with only two chromosome 3?

Reference no: EM133434444

Questions Cloud

Are the executive level increases fair when considering : In your opinion, are the executive level increases fair when considering the number of front-line workers who lost their jobs during the pandemic?

Doo you believe price transparency initiatives help : Doo you believe price transparency initiatives help the average American better understand the cost and quality of the services at various hospitals?

Steps of self-fulfilling prophecy : Identify each of the four steps of the self-fulfilling prophecy, and using example from your own life, share a time when you created a fulfilled prophecy.

Which does a large bureaucracy most likely do : Which does a large bureaucracy most likely do? Question 16 options: Encourage efficiency Cause feelings of dependency and inadequacy Influence neither way

What other sequencing technology would you use : What other sequencing technology would you use to verify the observed or hypothesized number of chromosomes for the LGM individual? Why

How can i critically analyse the case study farro biomed : How can I critically analyse the case study 'Farro Biomed: Effective Oversight When Leader Character Is a Risk' from an HR perspective and what are the main

How might social media be used to manage patients : What other examples have you seen? How effective have they been? How likely would you, as a patient, be to use these various methods of communication?

Compute for the ratio of the 2nd filial offspring : compute for the ratio of the 2nd filial offspring of the two given monohybrid crosses using a punnet square.

Write the sequence of the double-stranded dna : Write the sequence of the double-stranded DNA that encodes the following prokaryotic mRNA. Be sure to clearly indicate the template and non-template strands

User Account

All Pages