What of the would be a the best choice of a cloning vector

Assignment Help Biology
Reference no: EM13110329

You want to study the effect of a mutation in your favorite gene in a cell. You do not plan to purify the gene product, just look at the mutation in vivo. What of the following would be a the best choice of a cloning vector?

a. a vector that overexpresses YFG
b. a vector that allows you to fuse YFG with a 6xHIS tag
c. a single copy vector ( I think this is the right answer)
d. a multicopy vector

Reference no: EM13110329

Questions Cloud

Example of hypothesis test : Provide an example of a hypothesis test you could perform at work or at home. State what the Null and the Alternative hypotheses would be in your test.
When we graph the cfus/ml : When we graph the CFUs/ml and absorbance on the same graph, we would hope to see an upward trend for both. Sometimes the absorbance continues to rise after the CFUs/ml level off into the maximum stationary phase. What would be the cause of that?
Suggest strategies for evaluating hrm technologies : Suggest strategies for evaluating HRM technologies financial impact of talent management initiatives.
Determining student query-statistics : A wholesaler has recently developed a computerized sales invoicing system. Prior to implementing this system, a manual system was used. The distribution of the number of errors per invoice for the manual system is as follows
What of the would be a the best choice of a cloning vector : You want to study the effect of a mutation in your favorite gene in a cell. You do not plan to purify the gene product, just look at the mutation in vivo. What of the following would be a the best choice of a cloning vector?
Setting up study on depression treatments : Describe your selected research issue, problem, or opportunity. Formulate both a numerical and verbal hypothesis statement concerning your selected research issue, problem, or opportunity.
How many liters of hydrogen gas were produced : A volume of aqueous hydrochloric acid containing 0.500 mol of HCl and 0.100 mol of magnesium metal were combined and reacted to completion. How many liters of hydrogen gas were produced under STP conditions?
Experimental car bumper and repair incidents : An experimental bumper was designed to reduce damage in low-speed collisions. This bumper was installed on an experimental group of vans in a large fleet, but not on a control group.
Describe why ordinary least squares solution is not unique : Describe why ordinary least squares solution is not unique. What can you say about residuals of any solution? Is ridge regression solution unique? Explain why or why not?

Reviews

Write a Review

Biology Questions & Answers

  Radioactivity of the daughter dna molecules

The DNA molecule is permitted to replicate in a solution containing all four unlabeled deoxyribonucleoside tripohosphates and deoxyadenosine triphospage that is labeled with 32P.

  Why dna gyrase unwinds the dna double helix

Which of the following statements about DNA replication is true? Okazaki fragments are DNA fragments synthesized on the leading strand. Single?strand binding proteins stabilize the open conformation of the unwound DNA.

  What disorder does the man have

What disorder does this man have? Gastric secretions normally include about 10mmole/L potassium. How do you account for the low serum potassium in this patient.

  What are the disadvantages of obligate homeothermy

What are the disadvantages of obligate homeothermy, relative to the facultative homeothermy exhibited by many species of lizards and snakes?

  Case study on salmonella outbreaks in the u.s.

Case study on Salmonella Outbreaks in the U.S.

  Hypothetical breast cancer screening test:

If the preclinical prevalence of breast cancer increased in the screened population would you expect the positive predictive value to increase, decrease or remain unaltered? Why?

  Cell wall-nucleooid of bacterial cell-ionic-covalent bond

Describe in detail about cell wall and nucleooid of bacterial cell, their structure and composition.Explain the difference between ionic and covalent bonds.

  Color- blindness and normal vision

A color-blind woman marries a man with the normal color vision.  They have ten children, six boys and four girls. How many have normal vision? How many are color- blind?

  How will those hazards impact the population

How will those hazards impact the population who frequently visit the park and what measures could be put in place to reduce the hazard vulnerability.

  Diagnosing the ankylosing spondylitie

Explain how the ankylosing spondylitie is diagnosed and what are the diseases, which have symptoms that are similar to AS. Explain and list the organs which are affected by the Ankylosing Spondylitis.

  Define how anaerobic respiration plays a part

A number of major worldwide industries are based on anaerobic respiration in a lower form of organism.define how anaerobic respiration plays a part.

  Estimating diffusivity of solutes in a liquid

Determining Diffusivity of Solutes in a Liquid Compute the diffusion coefficient of a protein macromolecule bovine scrum albumin (BSA) at 23degreeC.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd