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The genome of virus SV40 is a circular double-stranded DNA molecule 5 kb long.
a. A 1 kb region is amplified by PCR for four cycles. What fraction of the total DNA does the target sequence constitute? Show your work.
b. After many generations of growth in medium containing 14N, the medium was replaced with new medium containing 15N. After five generations, what is the ratio of light:heavy DNA strands? Show your work.
c. If the number of thymine in the SV40 genome is 1830, what is the percentage of cytosine in the genome? Show your work.
A biologist was testing E. coli for thymidine requirement. She found a thy- point mutant and wanted to study reversion to thy+. She grew 20 cultures of the thy- mutant strain and plated them onto 20 plates that contained media
Crocodiles of the Nile often attack and kill large animals (such as wildebeest) by dragging them under the water and drowning them! Crocodiles can do this because they have the amazing ability to stay under the water for an hour or more.
Assume you are walking on the beach when you step on a shell and cut your foot. 4-cells of Vibrio vulnificus bacteria are transferred from shell and deposited deep inside the cut.
Compute the percentage reduction of bacteria accomplished by pasteurization of the milk sample.
Phenylalanine medium sometimes is prepared in broth form with a pH indicator similar to that used in Decarboxylase Medium so that increases in pH then can be detected by development of purple color. When testing an organism for the ability to deam..
The return of motility is sudden. What does this suggest about flagellar function in these organisms?
What would be the consequence if after ovulation, the primary oocyte fails to undergo its meiotic division to a secondary oocyte.
Why is DNA able to store large amounts of knowledge
Mycoplasmas are the simplest self-replicating (free-living) bacterium known. The Mycoplasma genitalium genome is 580,000 bases in length. Approximately how many genes does this bacterial genome encode.
When ten molecules of pyruvic acid are broken down completely to CO2 and H2O in the presence of oxygen, how many molecules of ATP will be produced?
How many times more hydroxide ions are there in a sloution with a pH of 9 then in a solution with a pH of 3.
A plant line with reduced fertility comes to attention of a plant breeder who observes that seed pods often contain a mixture of viable seeds that can be planted to create new plants.
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