Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
A few amino acids are post-translationally removed from the C-terminal end of the b-lactamase enzyme from B. imaginarium (i.e. - after translation and release from the ribosome, a protease chews off a few amino acids). The purified wildtype enzyme is 327 amino acids in length and the sequence of its C-terminal amino acids is shown below aligned with the C-terminal amino acids found in a mutant strain of B. imaginarium, which contains of a frameshift mutation that abolishes processing if its C-terminus. This frameshift mutant was caused by insertion or deletion of a single nucleotide. Use this information to answer the questions below.
wild-type: Ser Asn Ala Lys Asp Trp
mutant: Ser Asn Ala Ile Arg Leu Asp Trp Gln Met Lys
A. What was the mutational event that gave rise to the frameshift mutant? Determine precisely where the single nucleotide insertion or deletion occurred in the b-lactamase coding sequences.
B. What is the length of the wildtype enzyme prior to processing (how many amino acids)?
C. Deduce, as far as possible, the actual sequence of the C-terminus of the wild-type enzyme prior to processing.
Suppose that a dihybrid cross is made in which gene loci are autosomal, independently assorting, and incompletely dominant.
Determine the cell biology of periodontal ligament tissues (PDL) originating from cranial neural-crest-derived extomesenchymal cells.
What are the primary differences between animal cells, plant cells, and bacteria cells? What chacteristics do they share.
A patient lost a lot of blood during surgery and his blood pressure dropped from 120/80 to 90/50. Describe how the kidneys respond to this change in blood pressure.
Einstein once predicted that a sugar molecule glucose had a length of 1 nm. If glucose has a hexagonal structure, draw a picture of the 24 atoms in glucose.
An alpha helix of twenty to twenty-five amino acids is needed to span the plasma membrane of cells. Based on the dimensions of an alpha-helix, calculate the range in width of the membrane?
Genes A , B , and C are linked on a chromosome and found in the order A-B-C. Genes A and B recombine with a frequency of 10 percent.
The compound ouabain is a specific inhibitor of the active transport of Na+ out of the cell. For each of the following precesses in your body, would you expect the process to be sensitive to inhibition by ouabain?
What should we do to find out the consensus sequence of initial translation region around ATG for a specific species. Figure out the passive, active, and total stress components of an isometric experiment (label the axes) and provide simple diagrams..
Gennie is wild type. Below is a segment of her DNA. The top strand is the coding region, and the reading frame begins with the first codon that codes for met
According to our current knowledge of plant evolution, which group of organisms should feature mitosis most similar to that of land plants.
A person has MG what would be the expected effect on calcium levels near contractile proteins, following the arrival of an electrical impulse at those neuromuscular junctions.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd