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In mapping a large plasmid you notice that the restriction enzyme HaeII yields three bands, Pst I yields five bands and HaeII+PstI double digestion gives rise to six bands
A) What is the inconsistency of these data and what are two possible explanations?B) How could you distinguish between these possibilities from scrutiny of the gel electrophoresis results?C) You want to ligate the largest fragment generated by PstI with the largest fragment generated by HaeII. However the ends that are generated by the two enzymes are not complementary. What would you need to do in order to carry out the ligation?
In a cross of AABbCcdd x AaBbccDd what proportion of offspring will have the genotype of AABBCcdd? Please explain.
Determine which has a higher binding affinity for oxygen hemoglobin or myoglobin? Explain why this one must have a higher binding affinity for oxygen.
How the DNA mutation leads to the manifestation of the disease. Can the mutated DNA be removed in order to cure the disease? Why or why not?
Identify the importnat features of DNA polymerases and distinguish between the various DNA polymerases used in mlecular biology research.
Hematocrit values may be estimated from hemoglobin values by multiplying the hemoglobin value by three. Discuss how do the actual hematocrit values of your sample compare with the computed value obtained from the hemoglobin concentration?
Suppose that a dihybrid cross is made in which gene loci are autosomal, independently assorting, and incompletely dominant.
What is the free energy cost of pumping Ca2+ out of a cell when the cytoplasmic concentation is 0.4Um, the extracellular concentration is 1.5mM and the membrane potential is -60mV?
The seeds from Mendel's plants can produce tall, round and yellow dominant traits and their genes are independently assorted. The recessive genotypes dd, ww, and gg produce dwarf plants with wrinkled and green seeds respectively.
Gel filtration column was calibrated with blue dextran, its elution volume found to be 17.4 mL. Then, unknown protein and BSA were ran through the same column and eluted in 43 mL and thirty-six mL correspondingly.
Eye color is expressed thrugh two genes A and B. Dark brown eye color, A, is dominant over lighter brown a. Eye pigment, B, is dominant over lack of eye pigment, b.
Our digestive and urinary systems process our food and body waste to remain our bodies nourished and functioning. Poor diet and hygiene can compromise our digestive and urinary systems,
Explain the steps by which DNA sequencing is performed. Using at least one example, of how DNA sequencing has become a valuable tool in forensic medicine and/or biological research.
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