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The Voges Proskauer (VP) test detects a specific product of a mixed acid fermentation, which distinguishes the genus Escherichia from Klebsiella and Enterobacter. What is the biochemical basis for the VP test?
Describe the structure of atp and gtp. Describe how atp is produced during chemiosmosis. Illustrate two specific cellular processes that require atp and explain how atp is used in each process.
Hypothesize how recombination might adversely affect the functioning of an operon such as the lac operon or the trp operon. What might be the metabolic implications for an E. coli cell that experiences a disruption of one of these operons?
A man and woman are both of normal pigmentation and have a one child out of three who is albino (without melanin pigmentation). Albinism is an autosomal (not sex-linked) recessive trait. What are the genotypes of albino's parents.
Having studied the usefulness of restriction enzymes and the process of molecular cloning, propose a use for these two genetic technologies.
Briefly discuss the scientific and ethical considerations behind the cloning of whole organisms, especially humans.
The very simple genotype phenotype relationship of dominance, codominance and recessiveness has been complicated by issues of penetrance and epistatsis.
In a generalized transduction experiment, phages are collected from an E. coli donor strain of genotype cys+ leu+ thr+ and used to transduce a recipient of genotype cys- leu- thr-.
The correct folding of proteins is necessary to maintain healthy cells and tissues. Unfolded proteins are responsible for such neurodegenerative disorders as Alzheimer's, Huntington's, and Creutzfeld-Jacob disease.
A lacI+ lacO+ lacZ+ lacY+ Hfr strain is mated with an F- lacI- lacO+ lacZ- lacY- strain. In the absence of any inducer in the medium, beta-galactosidase is made for a short time after the lac region has been transferred. Explain why it is made and..
Curare, a poison extracted from a South American shrub, blocks the binding of Ach by muscle cells. What do you suppose would happen to your muscles, including the ones involved in breathing, if a toxic dose of curare entered your bloodstream?
Considering the nomenclature conventions used for fruit fly genetics, how are mutant and wild type alleles of genes distinguished when writing out a cross?
Why do many people oppose the insertion of genes into human patients to cure them of genetic disease ("gene therapy")?
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