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You have a cell suspension containing 4 * 10^5 cells/ml and want to seed these cells on microcarriers at a density of 1* 10^4 cells/cm^2. The average diameter of the microcarriers is 157.5um (the diameter range supplied by the vendor is 115-200um).What is the average surface area of the microcarriers?What number of microcarriers should you use to obtain an average surface area of 100cm^2?What volume of your cell suspension should be added to achieve the density given, using the surface area of 100cm^2?
Explain how to make 50 ml of the following 10X Gel electrophoresis loading buffer, 100 mM EDTA (pH 8.0), 0.1 percent and find mature mRNA.
Hematocrit values may be estimated from hemoglobin values by multiplying the hemoglobin value by three. Discuss how do the actual hematocrit values of your sample compare with the computed value obtained from the hemoglobin concentration?
For a specific desirable trait in a breed of sheep, VP is 28.6, VC is 11, and VA is 7.2. What is its broad sense heritability.
If the genotype of an organism is YySsTt, then yST would represent. the genotype of the offspring. a gamete of that parent organism. a possible phenotype of the offspring.
Discuss how much of a 10X buffer would I add to a reaction whose total volume will be 20?L?
Describe the structure and function of receptors involved in taste and olfaction. Describe how and why each of the following situations would affect someone's perception of flavor.
Briefly explain one aspect of ecology that you feel is relevant for all humans and discuss the difference between Clements and Gleason's views of how communities are structured?
If an individual was exercising at an exercise intensity of 1.5 liters of oxygen per minute, and had a mechanical efficiency of 20 percent, how many heat Calories would this person generate in 30 minutes?
What are the four most important elements necessary for life on this planet? Name the four most important groups of organic molecules for life? Give an example of each.
Determine which organ system are msot invovled with homeostasis and discuss the role of SNARE in exocytosis.
What is an animal? Include in your answer how animals are differentiated from other life forms. In particular, describe the similarities and differences between volvocine protozoa, members of the Phylum Porifera, and members of the Phylum Cnidaria th..
Compute the surface-area-to-volume ratio of a cube whose sides are 100 um in length to estimated the surface-area-to-volume ratio of the fern spore cell.
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