What is single-end and paired-end sequencing

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Reference no: EM132282121

1. Microarray analysis

For the following expression data in log2 transformation:

Name	0 hr	2 hr	4hr	6hr	8hr	10hr
geneA	0	3	3.58	4	3.58	3
geneB	0	1.58	2	2	1.58	1
geneC	0	-1.6	-2	-2	-1.2	-0.9
geneD	0	-3	-3.5	-3.8	-3.3	-3
geneE	0	2	3	2	0	1

1) Calculate Pearson Correlation Coefficient between each pair of genes.

2) Calculate the distance matrix as 1 - correlation.

3) Build a tree based on hierarchical clustering algorithm with average linkage.

2. High-throughput sequencing

Read the attached paper and answer the following questions:

Paper - Coming of age: ten years of next-generation sequencing technologies.

1) Explain WGS, Whole-exome and targeted sequencing, RNA-seq, and ChIP-seq.

2) Describe three template amplification strategies in few sentences.

3) What is single-end sequencing and what is paired-end sequencing?

4) What is real-time sequencing?

Attachment:- Paper.rar

Reference no: EM132282121

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