What is being measured in a microarray or rnaseq experiment

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Reference no: EM133391927

Questions:

1. What is being measured in a microarray or RNASeq experiment?

2. What can we learn from a microarray or RNASeq experiment?

3. Compare and contrast competitive (two-color) vs. comparative (one-color) microarray technologies. What are some advantages and disadvantages of each? What platforms use each of these technologies?

4. How does Affymetrix use perfect match and mismatch probes to improve quality of microarray results?

5. How are Illumina's BeadArray and Nanostring nCounter technologies similar to and different from Affymetrix gene chips?

6. Why is it important to have multiple sample replicates in a microarray or RNASeq experiment?

7. Define the following steps in microarray data processing:

8. How do you determine whether the difference in expression of a gene between two classes is significant?

9. What is multiple testing correction and why is it important in microarray or RNASeq gene expression studies?

10. What is a heatmap? What can we learn from a heatmap of gene expression data?

Reference no: EM133391927

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