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1) Describe how cDNA is produced, clearly indicating the relationship between a gene, an mRNA, and a cDNA?
2) What is an EST? How is an EST different from a genomic sequence? How is an EST different from a cDNA sequence? What is the EST database? What is a UniGene sequence (the Unigene database if you prefer)?
3)Describe a microarray and how it works? Indicate what is fixed to a support and what is used to probe the microarray. How does a microarray differ from a Northern blot?
Assume you hypothesize that an animal with a mutation that affects conversion of the b form of glycogen synthase to the a form could not produce glycogen at functional levels.
An individual heterozygous for 4-gene pairs AaBbCcDd was testcrossed to aabbccdd , and progeny were classified as follows,
I am conducting an activity assay to assess the Enzymatic Hydrolysis of 4-Nitrophenyl ?-D-Glucopyranoside by ?-D-Glucosidase. I am happy with the assay, though one of the components is bovine serum albumin ( 0.2% solution in phosphate buffer, to w..
In the aerials competition in skiing, the competitors speed down a ramp that slopes sharply upward at the end. The sharp upward slope launches them into the air, where they perform acrobatic maneuvers.
You have T cells that can respond to antigen A. Your spouse does not have T cells that respond to antigen A, so you offer to transfuse some of your T cells to your spouse. Why wouldn't it work? Be very specific.
Degrade a monoglyceride that has an 18-carbon fatty acid attached to it by Ester bonds. You will have to degrade the glycerol component followed by the fatty acid in presence of O2.
Cystic fibrosis is inherited with autosomal recessive allele individuals with autosomal dominant allele do not have the disease.
Explain the term convolution. Explain one of the image processing techniques in the spatial space for the medical imaging application.
Both endocrine and nervous system are major regulating system of the body; but nervous system has been compared to an airmail delivery system and the endocrine to pony express. Briefly explain the comparison.
What function of intermediate filaments have in eukaryotic cells.define structure of microfilaments and microfilament network.
Make an equation describe the relationship between DeltaG, equilibrium constant and prevailing concentrations of substrates and products of reactions.
Radiation therapy for cancer works because a cell's DNA is exposed during mitosis and most vulnerable to damage from radiation. Because cancer cells are uncontrollably undergoing mitosis.
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