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Restriction enzymes and elecrophoresis of DNA.
1. What does the undigested DNA control show us?
2. Did the enzymes completely digest all the DNA they were uncubated with? How do you know?
Assayed for LDH activity were five uL of a sample that was diluted 6 to 1. The activity in the reaction vessel, which has a volume of 1 mL, is 0.10 U.
In 1918, the tsar and tsarina of Russia and their five children were presumably assassinated and buried in an unmarked grave. Later, several women came forward and claimed that they were in fact Anastasia.
Two protein kinases, PK1 and PK2, work sequentially in intracellular signaling pathway. You construct cells that contain inactivating mutations in the genes that encode either PK1 or PK2
A membrane which is permeable to an uncharged molecule X separates two solutions. Solution A contains a 100 mM solution of X and B 10 mM. If the permeability constant for X is 5 cm/s.
A diffusion cell is constructed that consists of a thin membrane separating two solutions of identical volume. The donor solution contains a drug molecule of interest.
In labrador retreivers, black fur color is dominant to chocolate fur. A breeder crosses two heterozygous labs, resulting in a litter of nine puppies.
Discuss similarities and differences Rickettsia rickettsii and Chlamydia trachomatis with respect to structure, disease transmission, and pathogenesis.
Describe at least four functional (not structural) similarities between the purple bacterial photosynthetic reaction center (P860), and the PSI reaction center of green plants.
Why is the size of a bacterium more accurate for a negative stain than a direct staining method?
The reflex most likely responsible for prompting the urge to defecate that occurs within ten minutes of eating something would be.
Create four molecules of water clearly illustrating the correct orientation of the water molecules around the ammonia
You treat a partially purified preparation of protein with a reagent that breaks bonds between sulfur atoms. Which level(s) of protein structure are likely to be affected the most and WHY?
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