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You are doing a PCR that requires 1.6ng of DNA in 2uL of sample. You have 100uL of stock DNA with a concentration of 4ng/ul. Using reasonable pipetting volumes (ex. don't use too much of your sample but don't pipette an unreasonably small volume), how would you dilute your stock DNA sample to meet this criteria? Use the procedure in Exercise #3; show all formulae, variables, units and calculations.
What is a multiplex PCR? What differs in this reaction from a traditional PCR?
How are PCR products fluorescently labelled in the Identifiler Kit? Name four dyes used in this kit and their corresponding colours.
What standard is used during electropherogram interpretation to determine the size (in bp) of a peak given its electrophoretic mobility?
What reference standard is used during electropherogram interpretation to score alleles given the size of a DNA fragment?
Define incomplete 3' addition. How does this appear on an electropherogram?
What causes stutter? In the Identifiler kit, what is the most common size difference (in bp) between the true allele and the resulting stutter product? What does this difference represent?
Why is the pentose phosphate pathway more active in cells that are dividing than in those that are not? Is the difference in concentration of a substance across spaces, such as with powdered drink mix and water.
Human population doesn't follow a j-shaped curve since humans are able to consciously limit the number of offspring that they produce, which is what usually happens after a population industrializes.
Can you describe what happens during bacterial DNA replication (what are the stages, what happens during each one, what are the major types of proteins involved)?
Environments factors such as low folic acid, low vit b6, b12, inositol, metthionine and high homocysteine increases risk for NTCD. cysteine reduction in diets helps prevention also. What elese can be done to incrase or decrease the chances of a mo..
Underutilized or neglected plant species may have tremendous nutritional value and have agroecological niches so that they survive drought, unfertilized, saline or waterlogged soils better than current commercial species. What is it's unique nutri..
You have 100 ml each of 400 mg/ml sodium phosphate, 250 mg/ml sodium chloride, and 100 mg/ml potassium nitrate. You want to make one liter of a solution containing 10 mg/ml sodium phosphate, 10 mg/ml sodium chloride, and 10 mg/ml potassium nitrate..
You cross an M1M2 father with an M2M3 mother and ?nd out that the mean number of nosehairs in dissimilar marker genotypes in their offspring are as follows.
Explain the Preservation of Foods by Oscillating Magnetic Fields. Please provide a critical evaluation of this technology. Explain the possible mechanism of microbial inactivation by this approach.
q1. explain the process of dna extraction?what is the function of a restriction enzyme?for what purpose is gel
Assume that you have two lines of plants: one is A/A; B/B, the other is a/a; b/b. You cross the two and then self the F1 plants. With respect to these 2-genes:
An experimenter extracts and purifies act in filaments from ananimal cell. Using an appropriate visualization technique, herealizes that some actin filaments are very stable, somedepolymerize rapidly, and some depolymerize slowly.
When Tammy Byrd first entered a dark room she could not see well. In fact, she was momentarily blind. With time, however, her vision improved and was able to discern her surroundings. Explain this physiological phenomenon in terms of a light adapt..
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