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Design an enrichment culture medium and a protocol for the isolation and purification of a soil bacterium (e.g., Bacillus subtilis) from a sample of soil. Note possible contaminants and competitors. How will you adjust conditions of growth, and what conditions will be adjusted to enhance preferentially the growth of the Bacillus?
What was the hypothesis of this experiment? (Reminder: Hypothesis is a statement.) What question(s) was (were) the investigator asking.
find the momentum of a charge (3.2e-19) that moves perpendicular to a uniform magnetic field B of magnitude 0.20T and has radius of curvature of 4.0cm. Momentum=mv
When looking for a gene of interest, we often scan cDNA libraries. What is the difference between a cDNA and a genomic library? Can you think of situations in which we would probe a genomic library?
We are isolating DNA from a Marcian. THeir DNA turns out to be very very tiny, so we will use a very dense agarose gel to analyze the DNA. So, instead of the one percent agarose gels that we normally would use in lab,
Why is there a range of total ATP that can be produced through the process of glycolysis, the Krebs cycle, and oxidative phosphorylation?
What is an advantage of using GFP fusions to visualize specific proteins, instead of staining cells with fluorescently labelled probes that bind to the target protein.
Compute the volume of a red blood cell, assuming the blood cell is disk shaped with a diameter of ten um and a thickness of 3 um.
Suppose that the lengths of the double-helical regions and the lengths of the single-stranded regions in them vary within relatively tight limits.
Draw and label the sugar found in RNA, but not DNA. Number the carbons in the sugar.
Choose two organelles and compare them relative to structure and function. Describe what would be the specific probable effects on the cell if each of these organelles were lost.
The maltose transporter maltoporin Mr 49,913 spans the outer membrane of E. coli. It is comprised of eighteen successive antiparallel?
Describe the composition of gastric juice, identify the cell types responsible for secreting its components, evaluate the importance of each component in stomach activity, and explain regulation of gastric secretion and stomach motility?
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