What can deduce about the structure of this protein

Reference no: EM1339298

You have isolated a new protein and are in the process of characterizing it. Several pieces of data about the protein are presented below. What can you deduce about the structure of this protein at primary, secondary, tertiary, and quaternary levels.

A) The protein elutes in a void volume on a gel filtration column with a fractionation range of 30kDa to 300kDa

B) SDS-PAGE(reduced) shows the protein has three bands with molecular weights of about 215, 18, and 22kDa.

C) Following brief treatment of the original protein with chymotrypsin, the resulting protein sample now elutes from the gel filtration column as two peaks (250, 130kDa)

D) The 250kDa sample from C) runs as a single band at 125kDa on SDS PAGE (reduced) SDS PAGE (reduced) of the other sample from C) shows it to contain 3 bands 90,22, and 18kDa.

Reference no: EM1339298

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