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To 800 ml water, add 80 g of NaCl, 2.0g of KCl, 14.4 g Na2HPO4, and 2.4g KH2PO4; adjust the pH to 7.4 ; bring volume up to 1.0 liter with water.
1. What is the final concentration of KCl in 10x PBS? [The FW of KCl is 74.5]
A. 37.25
B. 0.0268
C.0.149
D. 149M
and what are the weak acid and the conjugate base in PBS?
A. HPO42 is the weak acid and H2PO4 is the conjugate base
B.H2PO4 is the weak acid and HPO4^2 is the conjugate base
C. NaCl is the weak acid and KCl is the conjugate base.
D. KCl and KH2PO4 make up the weak acid, NaCl and Na2HPO4 make up the conjugate base.
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