What are the final concentrations of dopa, citrate buffer

Reference no: EM13109915

calculate the final concentration of each of the components in the reaction mixture described in the folliwing experiment:

The enzyme tyrosinase is found in large concentrations in plant tissues. This enzyme is responsible for the browning of peeled potatoes and bruised fruits. In an assay to determine the activity of the tyrosinase in a potato extract, the following reaction mixture was prepared:

1ml of potato extract, 2ml of 5mM dopa (3,4-dihydroxyphenyalanine), 0.5ml of 20mM citrate buffer, 0.5ml of 1% hydroquinone and 2ml distilled water.

What are the final concentrations of dopa, citrate buffer, and hydroquinone in the reaction mixture?

Reference no: EM13109915

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