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1. Biologists frequently use phosphate-buffered solutions in experiments. You need to prepare a sodium phosphate solution at pH 6.7 by mixing NaH2PO4 and Na2HPO4 (pKa =7.2). What are the respective concentration of each of these substances if you want the final concentration of phosphate ([HPO4-2]+[H2PO4-] to be 150 mM?
2. Activity of the bacterial enzyme acetoacetate decarboxylase sharply declines below pH 6, suggesting there is an ionizable catalytic residue with pKa ~ 6. Using mutagenesis, this critical catalytic residue was determined to be Lys-115.
MCB354FA15_PS01
a) For catalytic Lys-115, what is the ratio of deprotonated to protonated side chain at pH 7.5?
b) The neighboring residue at position 116 is also lysine. Explain how this perturbs the pKa of the catalytic lysine residue.
In the F2 generation, you find that you have 235 flies displaying the wild type phenotype and 765 flies displaying XYZ phenotype. Which phenotype, Wild type or XYZ, is dominant.
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Classify each of the eight living things listed below into one of the two groups according to an important physical characteristic.
Write two page paper on Human physiology
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Recessive mutant alleles of an x-linked gene in Drosophila, crossveinless, prevent the development of supporting structures in the wings.
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