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Transcription of the pmocab operon

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  1. A gene includes a number of important sequences that ensure transcription and translation of the gene: promoter, initiation and termination sequences, RBS, start and stop codons. What is the role of each of these? For each of these, briefly describe its function (be sure to indicate whether it is important for transcription or for translation) and as the Baroness may question why she must understand such detail, indicate whether you think it would be important to include it in your explanation to the Baroness.
  2. Where are the important sequences from Question 2 above located? An image will be very helpful to show this. First you need to figure out the answers to the following:
  3. How many promoters would be necessary to allow transcription of the pmoCAB operon?
  4. How many mRNA molecules will be produced when this operon is transcribed?
  5. How many ribosome binding sites will be present on the RNA produced when the pmo operon is transcribed?
  6. How many start codons and stop codons will be present on the RNA produced when the pmo operon is transcribed?

Reference no: EM132391451

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