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The pentapeptide Val-Arg-Gln-Trp-Cys has N-terminal and C-terminal pKas of 9.5 and 4.5, respectively. The side chain pKa's are Arg 12.5 and Cys 8.0. a. Write this peptide sequence using single letter abbreviations. b. Draw its predominant chemical structure at pH 7.4, including charges. c. What fraction of the peptide will exist in the predominant form at pH 7.4? d. Calculate the pI of the peptide. e. If this pentapeptide is reacted with NEM (N-ethylmaleimide), what will be the pI of the product? f. To separate a mixture of the peptide and its NEM reaction product by ion exchange chromatography at pH 7.4, would you choose a CM- or DEAE-matrix? Explain why. g. Which peptide would elute first? Explain why. h. If the peptide and its NEM reaction product were separated by ESI TOF MS, which would reach the detector first? Explain why. i. If a solution of the pentapeptide, pH 6, has an absorbance of 0.5 at 280 nm in a standard 1-cm width spectrophotometer cuvette, what is the molar concentration of the peptide? What is its concentration in mg/ml? (For an estimate of molar absorptivity ?, in units of M-1 cm-1, see G & G Fig. 4-10 and round to one significant figure; for Mr values of amino acids, see lecture slide 4- 18.)
How humans populated the globe is a highly-debated question among paleoanthropologists. In 5 sentences or less, compare the "Out of Africa" theory to the "Multiregional Theory".
A 12-gram mixture of methane, CH4, and ethane, C2H6, is completely combusted in oxygen. If the total mass of CO2 produced is 34.8 grams, what was the approximate mass of the methane in the original mixture?
describe the first step involved in replication of each genome. Your description can include a diagram and should cover.
What information would you get from a western blot that you would not get from a northern blot when used to analyze the expression of the same gene?
A white lady, aged sixty, and she has lived in the USA all her life. She has three children, is 160 cm high and weighs ninety kg.
Arrange the following plants as to their complexity and explain your answer: angiosperms, viruses, bacteria, gymnosperms, ferns, mosses, algae, fungi?
You have three tubes containing solutions of the protein human chorionic gonadotrophin (HcG) in PBS. All three contain 2 ml. Tube A is at a concentration of 28 mg/ml. Tube B is at a concentration of 78 mg/ml. Tube C is at a concentration of 40 mg/..
You will take a culture of photosynthetic bacteria and bubble nitrogen gas through it to displace the dissolved in the solution. Why is it important to keep the bottle covered with foil while you do this?
Indicate the relationships between the afferent and efferent divisions of the peripheral nervous system, the somatic and autonomic nervous systems, and the sympathetic and parasympathetic divisions of the ANS.
A seven cm potato sliced is placed into an unknown solution. After the slice is immersed for one hour, the potato slice now measures six cm.
Membranes are essential components of both prokaryotic and eukaryotic cells. Identify three molecules or types of molecules that are components of the plasma membrane in eukaryotic cells. Discuss the structure and function of one of these componen..
Draw a genetic map for the location of these 4 genes on the chromosome. Be sure to show the map distances between the loci.
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