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The Entner-Doudoroff pathway, which is used by some microorganisms, including the talented Sinorhizobium meliloti:1) Glucose + ATP ? Glucose-6-phosphate + ADP2) Glucose-6-phospate + NADP+ ?6-Phosphogluconolactone + NADPH + H+3) 6-Phosphogluconolactone + H2O ?6-Phosphogluconate4) 6-Phosphogluconate ?2-Keto-3-deoxy-6-phosphogluconate5) 2-Keto-3-deoxy-6-phosphogluconate ?Glyceraldehyde-3-phosphate + Pyruvate6) Glyceraldehyde-3-phosphate + NAD+ + Pi ?1,3-Bisphosphateglycerate + NADH + H+7) 1,3-Bisphosphateglycerate + ADP ?3-Phosphoglycerate + ATP8) 3-Phosphoglycerate ?2-Phosphoglycerate9) 2-Phosphoglycerate ?Phosphoenolpyruvate + H2O10) Phosphoenolpyruvate + ADP ?Pyruvate + ATPA. Which of the reactions above are also found in the glycolysis pathway we are studying in class?B. How many (net) moles of ATP are produced per mole of glucose in the Entner-Doudoroff pathway? (Don't include any ATP made during the citric acid cycle or by oxidative phosphorylation.)C. Assuming the organism uses pyruvate dehydrogenase and the citric acid cycle for aerobic growth, how many (net) moles of ATP (or ATP + GTP) would be produced per mole of glucose by substrate-level phosphorylation as a result of the action of the Entner-Doudoroff pathway and the citric acid cycle?D. Identify the step(s) in the pathway above in which the metabolite is oxidized. Explain how you recognized that an oxidation-reduction reaction was taking place in this step (these steps).E. Assuming the organism has pyruvate dehydrogenase, how many moles of acetyl-CoA would be produced per mole of glucose when it is growing aerobically using the Entner-Doudoroff pathway? Explain how you got your answer.F. Again assuming the organism uses pyruvate dehydrogenase and the citric acid cycle for aerobic growth along with the Entner-Doudoroff pathway, how many moles of NADH plus NADPH would be produced per mole of glucose metabolized? Explain how you got your answer.
If there was mutation in a dna triplet that changed the first triplet from ctt to ctc, would that cause the problem with the resulting polypeptide.
Two plants in a cross were each heterozygous for two gene pairs whose loci are linked and twenty-five mu apart. Assuming that crossing over occurs during formation of both male and female gametes
If the genes were expressed in the cell, predict that signal would win out for the following combinations. Give details your reasoning.
If wind blows at 35 m/s^2 over the house, what is net force on the roof if its area is 240 square meters and the roof is flat? Show all work, with equation and substitution units.
Determine which of these would synthesize the enzymes for Histidine synthesis
Dr. Don McPhail and his colleagues hypothesized that the existence of the benthic and limnetic sticklebacks is the result of allopatric speciation.
Determine the regulations concerning venipuncture, drug administration, and IV medication. Discuss the possible repercussions that could arise when these standards are violated. How do the standards apply to ancillary health professionals?
There are several examples of eukaryotic cells. Algae, Fungi, Plants, and Animals are all comprises of eukaryotic cells.Select either a plant or an animal cell.
Import or export of solutes (chemiosmotic work) turning the flagellar motor (mechanical work) Synthesizing ATP via the F0F1 ATPase (chemical work; discussed next lecture) how is this energy generated or replenished.
While you measure the genotype frequencies of the population you get the following data. What will the frequency of Aa be in the next generation.
Explain the structure and functions of keratin. Does every layer of the epidermic contain keratin? Why or why not? Please provide a justification for your answer.
Assume if you were working with an organism like Arabidopsis, Drosophila, or any diploid organism that requires to use sexual reproduction to produce offspring.
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