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Q. After prolonged exposure to isoproterenol, that binds to b-adrenergic receptors and activates adenylate cyclase, cells become refractory and cease responding. To investigate this phenomenon, you treat cells for a variety of times with isoproterenol, wash it out, and next assay for b-adrenergic receptors by measuring binding of dihydroalprenolol, that is hydrophobic, and CGP-12177, which is hydrophilic. You find that CGP-12177 binding decreases in parallel to adenylate cyclase activity while dihydroalprenolol binding remains high. When you lyse isoproterenol-treated (desensitized) cells and fractionate membrane-enclosed vesicles by centrifugation through sucrose-density gradients, you find out that CGP-12177 binds only to vesicles derived from the plasma membrane (as indicated by the presence of the marker enzyme, 5'nucleotidase) whereas dihydroalprenolol binds to an extra population of vesicles as well.
a. Give an description for the differences in binding between CGP-12177 and dihydroalprenolol;
b. What do you think may be the basis for isoproterenol-induced desensitization in these cells?
What is the difference between lactococcus lactis and staphylococcus epidermis? I am doing a lab and I've found out that my bacteria are gram positive cocci that ferment lactose and mannitol. I have it narrowed down to lactococcs lactis and staphyloc..
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If populations are sustaining growth at r = 0.088 and the population in 2001 is 3000 individuals, in what year would snow geese be taken off an endangered species list.
Roges Staubauch has severe arthritis and has been taking prednisone for two months. He isn't feeling well, complains of repeated colds, and has become really puff in his appearance. What is the reason for this symptoms.
What is the possibility that their next child will show both defects? Demonstrate the work.
Assume n-butylmalonate is added to an aerobic suspension of kidney cells using glucose exclusively as fuel. Predict, with description, the effect of this inhibitor on.
Assuming that genotype essential to produce flat tails is homozygous recessive (such as bb) compute the frequency of the b allele in the squirrel population to two decimal places.
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Distinguish between mechanical advantages and disadvantages in lever systems and find out which kind of lever the doctor was using to test quadriceps muscle.
Explain a single procedure that could be used to isolate the Con A Receptor from this preparation. Propose two ways the photosynthesis process could be improved to fix more CO2 than normal and the plant productivity can be increased. Be specific abou..
Many infected animals are induced by the parasitic microsporidians to develop huge cells, known as xenomas, which are full of spores. Given their large size, what must be true of the xenomas.
With these anatomical features in mind, do research to discover an angiosperm species that has structural modifications to leaves, stems, roots, or flowers that result in adaptive functions. For in case, a California Barrel Cactus has spines in place..
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