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Q. A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to the mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in tetracycline resistance gene. This mixture is incubated for numerous hours, exposed to DNA ligase, and after that added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using the technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.
Bacteria containing a plasmid into which the eukaryotic gene has integrated will grow in what?
A mole of an assured compound contains 207.7 g of lead and 32.00g of oxygen. Without doing any calculations, tell whether a formula Unit of a compound contains more atoms of lead or more atoms of oxygen. Explain in details.
What is an advantage of using GFP fusions to visualize specific proteins, instead of staining cells with fluorescently labelled probes that bind to the target protein.
After a long scuba diving session on a Caribbean reef, Carl boards a plane to Dallas. He begins to feel pain in his elbow on the flight back to Dallas. What is happen to him and why? How does he require to be treated.
What genotypic and phenotypic proportions are expected in F2. Six platyfish with dissimilar tail spotting patterns live in eastern Mexico. Five of them can interbreed and produce fertile offspring. One of them cannot interbreed with the other five. W..
How to comprise the genotypes of both parents. The pronghorn of the United States and the saiga antelope of the central Asian steppes live in similar habitats and have alike adaptations. Which of the features would you be surprised to discover in a s..
Give an instance of a successful plant disease forecasting system and explain how it works. How plant diseases could be forecasted and what are the needs for that.
What do you think is the most probable mechanism of browning? If a patient's resting cardiac output is 5.6 l/min and on a stress test she elevated her heart rate to the highest of 176 beats/min with a stroke volume of 115 ml/beat, what is her cardiac..
Acetylcholine binds this membrane protein, which subsequently opens to allow sodium to enter the cell. Acetylcholine exerts its effect on what kind of transport protein.
Blood glucose was monitored weekly for all mice. After five weeks, there was no change in average body weight between groups.
Which of the follow is NOT a function of meiosis. Generation of genetic diversity by crossing over.
How could you explain the occurrence of birth defects (caused by altered genes) in children and grandchildren of WWII atomic bomb victims, while the victims themselves were only mildly affected.
Albinism is a condition that results from the lack of normal pigmentation. In humans, individuals with two recessive alleles at ALBINO (aa) locus are albino.
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