Reference no: EM132565225

1. You have a strain of Neurospora that is unable to synthesize histidine and thus requires H in the media in order to grow. You have isolated one revertant colony.

a. Predict the expected proportion of the progeny that would be h+ if you cross the colony with the original mutant colony and the reversion occurred by each of the following mechanisms:

i. Precise change of the mutated base back to its original base.

ii. A suppressor gene is mutated on a different chromosome

iii. A suppressor gene is mutated on the same chromosome but 10mu distant from the mutated gene.

b. The mutant colony is crossed to a wild-type Neurospora colony and the following data are collected. 95% of all asci scored are h+ but 5% are h-. Which mechanism in part a is consistent with these data? Explain why and what has happened on a molecular level.