Study protein-DNA interactions

Assignment Help Biology

Reference no: EM134019073

Question

Bacterial DNA has histone-like proteins, and so a similar protocol to ChIP can be used to study protein-DNA interactions in vivo in E. coli. You decide to study protein-DNA interactions in the lac operon of E. coli under different conditions. You use a crosslinker, an antibody that specifically binds the Lac Repressor protein and PCR primers to amplify the entire lac operon regulatory region.

Name one control you would use in this ChIP experiment and why it is important.You decide to make a reporter gene with 4 binding sites for a new transcription factor ZX2. Draw a simple schematic of the reporter gene below showing the 4 ZX2 binding sites, the reporter gene, and any other elements that are important to include. Include the name of the reporter gene that you choose.

Reference no: EM134019073

Questions Cloud

Explain the role of the restriction enzyme : Explain the role of the restriction enzyme used in the protocol in detecting the mutation.

Excess proteins in the urine : Explain why excess proteins in the urine of a pregnant woman are an indication of preeclampsia.

We began migrating across the globe : Modern humans evolved in Africa about 300,000 years ago. Around 100,000 years ago, we began migrating across the globe.

Activity on body composition and metabolic rate : The benefits of physical activity on body composition and metabolic rate include Multiple select question. increased bone density. increased lean muscle mass.

Study protein-DNA interactions : Bacterial DNA has histone-like proteins, and so a similar protocol to ChIP can be used to study protein-DNA interactions in vivo in E. coli.

Bacterial cell is attributed to which mechanism : The resistance to entire groups of antibiotics by removal from the bacterial cell is attributed to which mechanism below?

Exposed bacteria to uv light long enough to kill all of them : In lab, students exposed bacteria to UV light long enough to kill all of them. What would have happened if they had used x-rays instead?

What is source of acetyl-coa for fatty acid synthesis : What is the source of Acetyl-CoA for fatty acid synthesis? Where in the cell does fatty acid synthesis occur and which types of cells does fatty acid synthesis

Adaptive immune system by targeting specific antigens : Antimicrobial protein secreted by virus-infected cells is a(n) _ .Immunoglobulins, also called _ work in adaptive immune system by targeting specific antigens

User Account

All Pages