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Bradford Assay was performed with samples in triplicate. I got the standard curve and using three abosorbance from the plate reader for the samples and then I extrapolated the protein samples in ug for the triple values and then took a mean of them. Once I got the mean for the samples using the standard curve, I need to find the total protein concentraion of the samples.
How do I find that? I used 50 ul of the sample containing the protein. Let's say that for one of the sample I got the mean of 9 ug using the standard curve, how do I find the total protein concentration for this value (9 ug)? Do I divide 9 ug with 50 ul? Is there a formula I need to use. The total fraction is 500 uL.
In the turtles, brown shells and red legs are most prevalent forms of traits. Occassionally green shells and yellow legs are observed.
compute the isoelectric point of a protein. It would also be helpful if you could declare any constants (i.e. pH=5) in advance of both problems.
If repressor tetramer uses only two of its subunits to bind to an operator sequence, why is tetramer formation so important to the control of lac operon gene expression.
You start to have a scratchy feeling in the back of your throat. After a few days it hurts to swallow, so you go to doctor. The doctor tells you that you have strep throat.
lex A is an E. coli gene whose product regulates the transcription of certain target genes that are located at different positions in the bacterial chromosome.
The phosphorylation of ADP to form ATP stores approximately 7.3 kcal per mole o ATP. What is the approximate efficiency of cellular respiration for a "mutant" organism that produces only 29 mole of ATP for every mole of glucose oxidized, rather than ..
How the glucose and phospholipids would be distributed after you let the mixture settle for 30 minutes. How may this happen considering natural cleaning mechanism of the respiratory system.
On the morning of the 27th December 1996, Patsy Ramsey called the police to report her 5-year-old daughter, Jon Benet Ramsey, missing. The little girl had disappeared from her bedroom and a ransom note demanding one hundred and $8000.
When a yellow female Labrador retriever was mated with a brown male, half of puppies were brown and half were yellow. The same female, when mated to a brown male produced all brown puppies.
Traditionally, it was thought that nucleus was the control center of the cell. Recent discoveries though, indicate that the nucleus in eukaryotes is merely a repository of genetic data.
How will you find out the transcription factor binding sites in the whole genome. You have isolated a novel transcription factor. How would you decide the genes regulated by transcription factor in the whole genome.
Horn Sporophytes are horn-shaped. Horn Possess Gemmae. Can undergo sexual reproduction. Mosses what is the synapomorphy of hornworts, mosses and vascular plants? Stomata Pores that allow the uptake of CO2 and release of O2, do not close like other st..
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