Solublizing trypsin for trypsinization

Assignment Help Biology
Reference no: EM13530181

Why is it important to use a buffered salt solution that contains no Ca2+ or Mg2+, such as Hank's Balanced Salt Solution while diluting or solublizing trypsin for trypsinization?

Reference no: EM13530181

Questions Cloud

Isolated or parts of a multicellular body : Cells talk to each other, whether they are isolated or parts of a multicellular body. Using cells in an animal as your models, explain the general ways in which cell communicate.
Estimate the minimum amount of gasoline : You are preparing for a 5-day backpacking trip where you plan to use a gasoline cook stove for about 30 min each morning and night. Estimate the minimum amount of gasoline you should take on your trip
Find the average current that this cell phone draws : The battery for certain cell phone is rated at 3.70V. According to the manufacturer it can produce 3.15 X 10^4 J of electrical energy, find the average current that this cell phone draws when turned on
Involved inproducing and secreting a lipoprotein molecule : Describe the process involved inproducing and secreting a lipoprotein molecule from an animal cell.Do not explain HOW the protein or lipid is synthesized, but doexplain where they are synthesized, how and where the molecule isassembled, how it is pac..
Solublizing trypsin for trypsinization : Why is it important to use a buffered salt solution that contains no Ca2+ or Mg2+, such as Hank's Balanced Salt Solution while diluting or solublizing trypsin for trypsinization?
Determine the force required to just open the gate : A horizontal 2-m-diameter gate is located in the bottom of a water tank (5m depth). Determine the force F required to just open the gate
Find the maximum force the motor should exert : An elevator (mass 4600 ) is to be designed so that the maximum acceleration is 0.0650 What is the maximum force the motor should exert on the supporting cable
Find the average torque that the friction interation applied : A 6-kg bowling ball with a radius of 14 cm is spinning about a vertical axis (with its center of mass motionless) at an initial rate of 3 turns per second. What was the average torque that the friction interation applied to the bowling ball
Only this type of enzyme does not bind : Only this type of enzyme does NOT bind at least 2 substrates simultaneously. .The reaction below (L to R) is pyruvate----> acetyl CoA

Reviews

Write a Review

Biology Questions & Answers

  Mutation rate from normal to albino

Mutation rate from normal to albino is 1.3x10^-6 and selectionagainst albino form so only 9 in 10 survive to breed, what isequilibrium frequency of the albino allele

  How can you explain this observation

Hospital patients often become infected with resistant bacteria that are rarely found outside of the hospital environment. How can you explain this observation?

  Determine the genotypes

Parents of genotypes AABBCC and aabbcc (all loci are linked) are crossed to produce F1. An F1 individual is test crossed to produce the following F2 phenotypes:

  Which of these is not a characteristic of carbon

Which of these is not a characteristic of carbon.

  Which has the greatest affinity for electrons

Which has the greatest affinity for electrons.

  Explain why are algae significant in nature how are algae

why are algae important in nature? how are algae fungi and lichens related and how is this relationship defined?

  Sum up any possible hereditary disease you can recognize

write a brief 2 page typed double-spaced summary about your genogram.summarize any possible hereditary disease you can

  How would changing the histidine codons to alanine codon

A number of bacterial biosynthetic operons have been examined in Escherichia coli and Salmonella typhimurium, many of which show attenuation very similar to that seen in the tryptophan system in E. coli.

  What is the probability that this couple first child

Assume that a man with dentinogenesis imperfecta, whose father had the disease but whose mother had normal teeth, married a woman with normal teeth. They have six children.What is the probability that this couple's first child will be a male with ..

  What was the unknown substance added

The [H+] of a solution is 1.0 x 10-5 M. After the addition of an unknown substance, the [OH-] was 1.0 x 10-3M. What was the unknown substance added?

  How does radiation cause biological harm to bacteria

Based on their respective oxygen requirements, in which regions of the thioglycollate tubes do you expect to see growth of Microccocus luteus, Proteus vulgaris, and Clostridium perfringens?

  Select one age-related gastrointestinal disease how would

choose one age-related gastrointestinal disease. how would you describe the disease and why is this age group more

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd