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Suppose a broth has 436 million bacteria per milliliter. A serial dilution is made using three bottles of 99 milliliters each. 1 mL is transferred from the sample to the first bottle; 1 mL is transferred from the first bottle to the second; and then 1 mL is transferred from the second bottle to the third. From the third bottle 0.1 mL is transferred to a pour plate. How many colonies would you expect to find on this plate? Show the work.
Assume that you isolated Staphylococcus aureus from your skin. How would you determine whether it is penicillin-resistance?
A two year old boy is being seen through a hematologist. The child's symptoms include the sudden onset of high fevers, thrombocytopenia, epistaxis.
What will be appearance of the offspring of a cross of the F1 peaches back to that smooth,oval glanded parent. How several diploid Drosophila cells would be needed to have 1 picomole of each allele.
Provide two examples where the nature of the DNA sequence itself is the basis for an increased rate of mutation. Explain why these sequences predispose to mutations.
In this example, for every base designated Q, there is twice that amount of base R; for every base Z, there is twice that amount of base S. If molecule contains 33.33 percent R, what percentage of Z would you expect.
The membrane vesicles are next washed, the membranes solubilized and the proteins denatured and analyzed by SDS gel electrophoresis and Western blotting using polyclonal antibodies against glycophorin A, an erythrocyte plasma membrane protein.
Calculate the physiological standard free energy change for the combined reactions in glycolysis converting 1,3-bisphosphoglycerate to pyruvate.
What are the probable genotypes of all the parents. What specific benefits will cellular organelles have if enclosed by a cell membrane alike to the plasma membrane.
Linear double stranded DNA- sample LSU1- is digested with 2 restriction endonucleases (separately and together) and the following bands are observed after electrophoresis ( quantities represent length in bp): BamH1:1,9 and 13.5;EcoR1: 3, 3.5, 4, a..
Nucleoplasmin is a nuclear protein. Protein was divided into two segments and linked to the same large cytoplasmic protein, generating two fusion proteins
Explain how this exchange of materials helps support homeostasis within the cell, and allows for life functions within the cell.
When examining geology of a region for potential useable aquifers, what characteristics or factors would you consider? Also, taking into account certain natural and human factors, which areas would you avoid.
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