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A plasmid 3 Kb in length contains a gene for ampicillin resistance and a gene for tetracycline resistance. The plasmid has a single site for each of EcoRI, Bg/II, and PstI. If DNA is cloned into the EcoRI site, resistance to either antibiotic is not affected. DNA cloned into the PstI site eliminates ampicillin resistance.DNA cloned into the Bg/II site abolishes terracycline resistance. Complete double digestion fragment produced are as follows: EcoRI + PstI=2.3 and 0.7 Kb; EcoRI + Bg/II=2.77 and 0.3 kb. Draw a restriction map of the plasmid, showing the locations of the antibiotic resistance genes and the restriction enzyeme sites.
Determine which phase of the ventricular myocyte action potential would you expect to be most affected by verapamil? Why? What would the end result of this be?
Why was the discovery of microsphere and coacervates an important contribution to understanding of how life might have originated on earth .
Compute the number of molecules of gramicidin in eight ml of a 50nM solution. Suppose the same fraction of gramicidin remains as part of the membrane as the fraction of lipids.
Trace the pathway this particle would probably take while moving from outside the cell to inside. What organelles will it pass through? Where will it be located within these organelles.
Use methanogenesis as an example to explain syntrophy and explain in energetic perspective why it is so important to have both organisms live together.
Suppose i f an individual with genotype pQ/Pq heterozygous for both loci in trans arrangement of alleles is crossed with an individual of genotype PQ/pq.
Drugs known as nucleoside analogs (NA) act against RT, whereas drugs called protease inhibitors (PI) act against PR.
Determine where the chain would be more likely to show beta bends or reverse turns.explain where one would fin cross-linked disulfide bonds.
Tracey had been slightly concerned about trace glucose that was found in Max's urine 6-hours after his exercise until she found that he had eaten an entire large pizza an hour before the urinalysis.
Which piece of evidence below (a-d) best supports the hypothesis that protein structure and function are correlated?
Estimate how many photons of light are minimally required to build one glucose molecule in photosynthesis and describe your answer
A microbiologist inoculates a growth medium with 200 bacterial cells/ml. If the generation time of the species is 1.5 hours. How many generations will it take for the culture to contain more than 6,000 cells/ml? How long, in hours, will it be befo..
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